TIA model is attainable in Wistar rats by intraluminal occlusion of the MCA for 10 min or shorter

Simple item page
dc.contributor.authorDurukan Tolvanen A.
dc.contributor.authorTatlisumak E.
dc.contributor.authorPedrono E.
dc.contributor.authorAbo-Ramadan U.
dc.contributor.authorTatlisumak T.
dc.date.accessioned2024-07-22T08:10:57Z
dc.date.available2024-07-22T08:10:57Z
dc.date.issued2017
dc.description.abstractTransient ischemic attack (TIA) has received only little attention in the experimental research field. Recently, we introduced a TIA model for mice, and here we set similar principles for simulating this human condition in Wistar rats. In the model: 1) transient nature of the event is ensured, and 2) 24 h after the event animals are free from any sensorimotor deficit and from any detectable lesion by magnetic resonance imaging (MRI). Animals experienced varying durations of ischemia (5, 10, 12.5, 15, 25, and 30 min, n = 6–8 per group) by intraluminal middle cerebral artery occlusion (MCAO). Ischemia severity and reperfusion rates were controlled by cerebral blood flow measurements. Sensorimotor neurological evaluations and MRI at 24 h differentiated between TIA and ischemic stroke. Hematoxylin and eosin staining and apoptotic cell counts revealed pathological correlates of the event. We found that already 12.5 min of ischemia was long enough to induce ischemic stroke in Wistar rats. Ten min or shorter durations induced neither gross neurological deficits nor infarcts visible on MRI, but histologically caused selective neuronal necrosis. A separate group of animals with 10 min of ischemia followed up to 1 week after reperfusion remained free of infarction and any MRI signal change. Thus, 10 min or shorter focal cerebral ischemia induced by intraluminal MCAO in Wistar rats provides a clinically relevant TIA the rat. This model is useful for studying molecular correlates of TIA. © 2017 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.brainres.2017.03.010
dc.identifier.issn00068993
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15462
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectBrain Ischemia
dc.subjectCerebrovascular Circulation
dc.subjectDisease Models, Animal
dc.subjectInfarction, Middle Cerebral Artery
dc.subjectIschemic Attack, Transient
dc.subjectMale
dc.subjectNecrosis
dc.subjectNeurons
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReperfusion
dc.subjectStroke
dc.subjectTime Factors
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectapoptosis
dc.subjectArticle
dc.subjectblood flowmetry
dc.subjectbrain blood flow
dc.subjectbrain ischemia
dc.subjectbrain size
dc.subjectcell count
dc.subjectcomparative study
dc.subjectcontrolled study
dc.subjectdisease duration
dc.subjectdisease severity
dc.subjectfollow up
dc.subjecthistopathology
dc.subjectmale
dc.subjectmiddle cerebral artery occlusion
dc.subjectnerve cell necrosis
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance imaging
dc.subjectpriority journal
dc.subjectrat
dc.subjectrectum temperature
dc.subjectreperfusion
dc.subjecttransient ischemic attack
dc.subjectTUNEL assay
dc.subjectweight reduction
dc.subjectanimal
dc.subjectbrain circulation
dc.subjectcerebral artery disease
dc.subjectcerebrovascular accident
dc.subjectcomplication
dc.subjectdisease model
dc.subjectnecrosis
dc.subjectnerve cell
dc.subjectpathology
dc.subjectphysiology
dc.subjectprocedures
dc.subjecttime factor
dc.subjecttransient ischemic attack
dc.subjectveterinary
dc.subjectWistar rat
dc.titleTIA model is attainable in Wistar rats by intraluminal occlusion of the MCA for 10 min or shorter
dc.typeArticle

Files

Collections

Scopus Koleksiyonu