Detection of Leishmania major and Leishmania tropica in domestic cats in the Ege Region of Turkey

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dc.contributor.authorPaşa S.
dc.contributor.authorTetik Vardarli A.
dc.contributor.authorErol N.
dc.contributor.authorKarakuş M.
dc.contributor.authorTöz S.
dc.contributor.authorAtasoy A.
dc.contributor.authorBalcioğlu T.C.
dc.contributor.authorEmek Tuna G.
dc.contributor.authorErmiş T.V.
dc.contributor.authorErtabaklar H.
dc.contributor.authorÖzbel Y.
dc.date.accessioned2024-07-22T08:13:00Z
dc.date.available2024-07-22T08:13:00Z
dc.date.issued2015
dc.description.abstractLeishmaniosis is a group of diseases caused by different species of Leishmania parasites in mammalian species. The aim of the present study was to investigate the presence of Leishmania spp. DNA in cats using real time polymerase chain reaction (RT-PCR) assays targeting internal transcribed spacer (ITS1) and heat-shock protein 70 gene (Hsp70) regions with Leishmania species-specific primers and probes. Blood samples were collected from 147 cats (73 female; 74 male) in the endemic regions for zoonotic visceral leishmaniasis in the western provinces of Turkey and analyzed using two RT-PCR assays. Additionally, Hsp70 RT-PCR products were sequenced. ELISA assays for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) were also carried out for 145 of the 147 samples.Overall, 13/147 (8.84%) cats were positive for Leishmania by RT-PCR (4 L. major and 9 L. tropica). FIV and FeLV antibody and/or antigen was detected in 4 and 5 cats among Leishmania DNA positives, respectively. To the best of our knowledge, this study is the first to investigate and report the presence of L. major and L. tropica infections in a large group of domestic cats in Turkey. The results obtained indicate that species identification of Leishmania is essential for epidemiological understanding and that clinical signs alone are not indicative for leishmaniosis in cats, as it is in dogs. This study suggests that extensive research should be carried out in cat populations in order to fully understand the role of cats in the epidemiology of the disease. © 2015 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.vetpar.2015.07.042
dc.identifier.issn03044017
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/16254
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAnimals
dc.subjectAntibodies, Viral
dc.subjectCat Diseases
dc.subjectCats
dc.subjectCoinfection
dc.subjectFemale
dc.subjectImmunodeficiency Virus, Feline
dc.subjectLeishmania major
dc.subjectLeishmania tropica
dc.subjectLeishmaniasis, Cutaneous
dc.subjectLentivirus Infections
dc.subjectLeukemia Virus, Feline
dc.subjectMale
dc.subjectRetroviridae Infections
dc.subjectTumor Virus Infections
dc.subjectTurkey
dc.subjectDNA
dc.subjectheat shock protein 70
dc.subjectinternal transcribed spacer 1
dc.subjectvirus antibody
dc.subjectArticle
dc.subjectblood sampling
dc.subjectcat disease
dc.subjectcontrolled study
dc.subjectdomestic cat
dc.subjectenzyme linked immunosorbent assay
dc.subjectFeline immunodeficiency virus
dc.subjectFeline leukemia virus
dc.subjectfemale
dc.subjectgene
dc.subjectgene sequence
dc.subjectHsp70 gene
dc.subjectITS1 gene
dc.subjectLeishmania major
dc.subjectLeishmania tropica
dc.subjectleishmaniasis
dc.subjectmale
dc.subjectmolecular probe
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectparasite identification
dc.subjectprevalence
dc.subjectreal time polymerase chain reaction
dc.subjectTurkey (republic)
dc.subjectanimal
dc.subjectblood
dc.subjectcat
dc.subjectCat Diseases
dc.subjectcomplication
dc.subjectisolation and purification
dc.subjectLeishmaniasis, Cutaneous
dc.subjectLentivirus Infections
dc.subjectmixed infection
dc.subjectparasitology
dc.subjectRetroviridae Infections
dc.subjectTumor Virus Infections
dc.subjectTurkey
dc.subjectveterinary
dc.subjectvirology
dc.titleDetection of Leishmania major and Leishmania tropica in domestic cats in the Ege Region of Turkey
dc.typeArticle

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