Oleuropein Has Modulatory Effects on Systemic Lipopolysaccharide-Induced Neuroinflammation in Male Rats

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dc.contributor.authorŞahin S.
dc.contributor.authorŞahin E.
dc.contributor.authorEsenülkü G.
dc.contributor.authorRenda G.
dc.contributor.authorGürgen S.G.
dc.contributor.authorAlver A.
dc.contributor.authorAbidin İ.
dc.contributor.authorCansu A.
dc.date.accessioned2024-07-22T08:01:22Z
dc.date.available2024-07-22T08:01:22Z
dc.date.issued2024
dc.description.abstractBackground: Neuroinflammation induced by systemic inflammation is a risk factor for developing chronic neurologic disorders. Oleuropein (OLE) has antioxidant and anti-inflammatory properties; however, its effect on systemic inflammation-related neuroinflammation is unknown. Objectives: This study aimed to determine whether OLE protects against systemic lipopolysaccharide (LPS)-induced neuroinflammation in rats. Methods: Six-wk-old Wistar rats were randomly assigned to 1 of the following 5 groups: 1) control, 2) OLE-only, 3) LPS + vehicle, 4) OLE+LPS (O-LPS), and 5) a single-dose OLE + LPS (SO-LPS group). OLE 200 mg/kg or saline as a vehicle was administered via gavage for 7 d. On the seventh day, 2.5 mg/kg LPS was intraperitoneally administered. The rats were decapitated after 24 h of LPS treatment, and serum collection and tissue dissection were performed. The study assessed astrocyte and microglial activation using glial fibrillary acidic protein (GFAP) and CD11b immunohistochemistry, nod-like receptor protein-3, interleukin (IL)-1β, IL-17A, and IL-4 concentrations in prefrontal and hippocampal tissues via enzyme-linked immunosorbent assay, and total antioxidant/oxidant status (TAS/TOS) in serum and tissues via spectrophotometry. Results: In both the O-LPS and SO-LPS groups, LPS-related activation of microglia and astrocytes was suppressed in the cortex and hippocampus (P < 0.001), excluding cortical astrocyte activation, which was suppressed only in the SO-LPS group (P < 0.001). Hippocampal GFAP immunoreactivity and IL-17A concentrations in the dentate gyrus were higher in the OLE group than those in the control group, but LPS-related increases in these concentrations were suppressed in the O-LPS group. The O-LPS group had higher cortical TAS and IL-4 concentrations. Conclusions: OLE suppressed LPS-related astrocyte and microglial activation in the hippocampus and cortex. The OLE-induced increase in cortical IL-4 concentrations indicates the induction of an anti-inflammatory phenotype of microglia. OLE may also modulate astrocyte and IL-17A functions, which could explain its opposing effects on hippocampal GFAP immunoreactivity and IL-17A concentrations when administered with or without LPS. © 2024 American Society for Nutrition
dc.identifier.DOI-ID10.1016/j.tjnut.2024.02.017
dc.identifier.issn00223166
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11422
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAnimals
dc.subjectAnti-Inflammatory Agents
dc.subjectAntioxidants
dc.subjectHippocampus
dc.subjectInflammation
dc.subjectInterleukin-17
dc.subjectInterleukin-1beta
dc.subjectInterleukin-4
dc.subjectIridoid Glucosides
dc.subjectLipopolysaccharides
dc.subjectMale
dc.subjectMicroglia
dc.subjectNeuroinflammatory Diseases
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectCD11b antigen
dc.subjectcryopyrin
dc.subjectglial fibrillary acidic protein
dc.subjecthydrogen peroxide
dc.subjectinterleukin 17
dc.subjectinterleukin 1beta
dc.subjectinterleukin 4
dc.subjectoleuropein
dc.subjectantiinflammatory agent
dc.subjectantioxidant
dc.subjectinterleukin 17
dc.subjectinterleukin 1beta
dc.subjectinterleukin 4
dc.subjectiridoid
dc.subjectlipopolysaccharide
dc.subjectoleuropein
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantibody labeling
dc.subjectantioxidant activity
dc.subjectArticle
dc.subjectastrocyte
dc.subjectcolorimetry
dc.subjectcontrolled study
dc.subjectdentate gyrus
dc.subjectenzyme linked immunosorbent assay
dc.subjectfemale
dc.subjectgranular cell
dc.subjecthippocampal CA3 region
dc.subjecthippocampal tissue
dc.subjecthippocampus
dc.subjectimmunity
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectlipopolysaccharide-induced neuroinflammation
dc.subjectmale
dc.subjectmicroglia
dc.subjectnonhuman
dc.subjectoxidation reduction state
dc.subjectprefrontal cortex
dc.subjectrat
dc.subjectspectrophotometry
dc.subjectstratum pyramidale
dc.subjectthin layer chromatography
dc.subjectanimal
dc.subjectinflammation
dc.subjectmetabolism
dc.subjectnervous system inflammation
dc.subjectWistar rat
dc.titleOleuropein Has Modulatory Effects on Systemic Lipopolysaccharide-Induced Neuroinflammation in Male Rats
dc.typeArticle

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