Comparative phytochemical studies on the roots of Polygala azizsancarii and P. peshmenii and neuroprotective activities of the two xanthones

Simple item page
dc.contributor.authorÇalış İ.
dc.contributor.authorBecer E.
dc.contributor.authorÜnlü A.
dc.contributor.authorUğurlu Aydın Z.
dc.contributor.authorHanoğlu A.
dc.contributor.authorVatansever H.S.
dc.contributor.authorDönmez A.A.
dc.date.accessioned2024-07-22T08:02:55Z
dc.date.available2024-07-22T08:02:55Z
dc.date.issued2023
dc.description.abstractSix known sucrose mono-, di- and triesters and five xanthone derivatives were isolated from the roots of Polygala peshmenii Eren, Parolly, Raus & Kürschner which is a narrow species endemic to Türkiye. Among the xanthones, 1,7-dihydroxy-2,3-methylenedioxy-5,6-dimethoxy-xanthone is an undescribed compound isolated for the first time from a natural source. The studies on the roots of P. azizsancarii Dönmez have resulted in the isolation of four known compounds including sucrose mono-, di- and triesters. The structures of the sucrose esters and xanthones isolated from P. azizsancarii and P. peshmenii were established by spectroscopic methods, including 1D-NMR (1H NMR, 13C NMR, DEPT-135), 2D-NMR (COSY, NOESY, HSQC, HMBC). Neuroprotective activities of two xanthones, 1,3,6-trihydroxy-2,5,7-trimethoxyxanthone and 3-O-β-D-glucopyranosyloxy-1,6-dihydroxy-2,5,7-trimethoxyxanthone isolated from the roots of P. azizsancarii were evaluated in vitro using in a cellular model of Alzheimer's disease. SKNAS human neuroblastoma cells were used in the study and treated with different consecrations of Aβ₂₅₋₃₅ oligomer for up to 48 h. Cell viability was evaluated using MTT assay. The distribution of β-amyloid, α-synuclein, tau, JAK2, STAT3, caspase 3 and BMP-2 were investigated using indirect immunoperoxidase staining. Our results suggested that both xanthones control tau aggregation with no effect on β-amyloid plaque formation. In addition, for neuronal pathophysiology in AD cell model, decreased distributions of JAK/STAT3 and BMP2 signaling pathways were demonstrated, therefore they play a role in the protective effect on neurons in neurodegenerative disease. A significant decrease in caspase 3 immunoreactivity was detected after the administration of both compounds in AD cells. Therefore, both compounds control neuronal pathophysiology and rescue cell death in AD disease. © 2023 Elsevier Ltd
dc.identifier.DOI-ID10.1016/j.phytochem.2023.113650
dc.identifier.issn00319422
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12052
dc.language.isoEnglish
dc.publisherElsevier Ltd
dc.subjectCaspase 3
dc.subjectHumans
dc.subjectMagnetic Resonance Spectroscopy
dc.subjectNeurodegenerative Diseases
dc.subjectPlant Roots
dc.subjectPolygala
dc.subjectSucrose
dc.subjectXanthones
dc.subjectcaspase 3
dc.subjectsucrose
dc.subjectxanthone derivative
dc.subjectchemistry
dc.subjectdegenerative disease
dc.subjecthuman
dc.subjectnuclear magnetic resonance spectroscopy
dc.subjectplant root
dc.subjectPolygala
dc.titleComparative phytochemical studies on the roots of Polygala azizsancarii and P. peshmenii and neuroprotective activities of the two xanthones
dc.typeArticle

Files

Collections

Scopus Koleksiyonu