Synergistic role of three dimensional niche and hypoxia on conservation of cancer stern cell phenotype

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dc.contributor.authorGorgun, C
dc.contributor.authorOzturk, S
dc.contributor.authorGokalp, S
dc.contributor.authorVatansever, S
dc.contributor.authorGurhan, SID
dc.contributor.authorUrkmez, AS
dc.date.accessioned2025-04-10T10:35:57Z
dc.date.available2025-04-10T10:35:57Z
dc.description.abstractHypoxia is a pathalogical condition in which tissues are deprived of adequate oxygen supply. The hypoxia effect on tumors has a critically important role on maintenance of cancer stem cell phenotype. The aim of this study is to investigate the effects of hypoxia on cancer stem cells on three dimensional (3D) in vitro culture models. Osteosarcoma stem cells characterized by CD133 surface protein were isolated from osteosarcoma cell line (SaOS-2) by magnetic-activated cell sorting (MACS) technique. Isolated CD133(+) and CD133(-) cells were cultivated under hypoxic (1% O-2) and normoxic conditions (21% O-2) for 3 days. For the 3D model, bacterial cellulose scaffold was used as the culture substrate. 3D morphologies of cells were examined by scanning electron microscopy (SEM); RT-PCR and immunocytochemistry staining were used to demonstrate conservation of the cancer stem cell phenotype in 3D environment under hypoxic conditions. Cell viability was shown by MTT assay on 3. and 7. culture days. This study is seen as an introduction to develop a 3D hypoxic cancer stem cell based tumor model to study CSC behavior and tumor genesis in vitro. (C) 2016 Published by Elsevier B.V.
dc.identifier.e-issn1879-0003
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/20.500.14701/41884
dc.language.isoEnglish
dc.titleSynergistic role of three dimensional niche and hypoxia on conservation of cancer stern cell phenotype
dc.typeArticle; Proceedings Paper

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