Glycoconjugate histochemistry of mucous glands in the skin of metamorphosing Bufo viridis

Simple item page
dc.contributor.authorSahin M.
dc.contributor.authorGumus A.
dc.contributor.authorBalcan E.
dc.date.accessioned2024-07-22T08:22:24Z
dc.date.available2024-07-22T08:22:24Z
dc.date.issued2008
dc.description.abstractMucins are the major glycoprotein secretions of mucous glands and display important functions in amphibian skin such as regulation of water homeostasis and mechanical and chemical protection. In the present study, we evaluated the glycoconjugate contents of developing mucous glands on dorsal regions of metamorphosing Bufo viridis (Amphibia: Anura) tadpoles using an alcian blue-PAS panel and lectin histochemistry. All the conical cells of mucous glands showed weak positivity for alcian blue in 0.025 M MgCl2 at pH 5.7 but only a few cells were positive for 0.3 M MgCl2 at the same pH. In addition, all the conical cells of mucous glands were negative for alcian blue at pH 2.5. In lectin histochemistry, conical cells reacted strongly with Galanthus nivalis agglutinin (GNA), Datura stramonium agglutinin (DSA) and peanut agglutinin (PNA), weakly with Maackia amurensis leucoagglutinin (MAL). These results suggest that they express predominantly mannose, galactose and partially α(2→3)-linked sialic acid containing glycoconjugates. We concluded that dorsal mucous glands of metamorphosing Bufo viridis tadpoles contain at least two different conical cell types and glycoconjugate heterogeneity of mucous glands may be related with different functions of mucins. © 2008 Versita.
dc.identifier.DOI-ID10.2478/s11756-008-0071-6
dc.identifier.issn13369563
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19071
dc.language.isoEnglish
dc.subjectAmphibia
dc.subjectAnura
dc.subjectArachis hypogaea
dc.subjectBufo viridis
dc.subjectDatura stramonium
dc.subjectGalanthus
dc.subjectGalanthus nivalis
dc.subjectMaackia
dc.subjectMaackia amurensis
dc.titleGlycoconjugate histochemistry of mucous glands in the skin of metamorphosing Bufo viridis
dc.typeArticle

Files

Collections

Scopus Koleksiyonu