Post-ischemic leakiness of the blood-brain barrier: A quantitative and systematic assessment by Patlak plots

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dc.contributor.authorAbo-Ramadan U.
dc.contributor.authorDurukan A.
dc.contributor.authorPitkonen M.
dc.contributor.authorMarinkovic I.
dc.contributor.authorTatlisumak E.
dc.contributor.authorPedrono E.
dc.contributor.authorSoinne L.
dc.contributor.authorStrbian D.
dc.contributor.authorTatlisumak T.
dc.date.accessioned2024-07-22T08:21:40Z
dc.date.available2024-07-22T08:21:40Z
dc.date.issued2009
dc.description.abstractThe Patlak plot analysis of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) allows estimation of blood-brain barrier (BBB) leakage following temporary focal cerebral ischemia. Thus far, a systematic and quantitative in vivo evaluation of post-ischemic BBB leakage is lacking. Here, using DCE-MRI and the Patlak plot method, we quantitatively assessed BBB leakage in rats at the following time-points after reperfusion: 25 min, 2, 4, 6, 12, 18, 24, 36, 48, and 72 h, and 1, 2, 3, 4, and 5 weeks. Sham-operated animals served as controls. Data collected for each time-point were: the blood-to-brain transfer rate constant (Ki) of the contrast agent gadolinium, distribution volume (Vp), ischemic lesion volume, and apparent diffusion coefficient (ADC) values. Compared to controls, Ki, measured at all time-points, except for 5 weeks, appeared significantly different (p < 0.001). At several time-points (25 min, 48 and 72 h, 4 and 5 weeks), Vp was similar compared to that of controls, but for the remaining groups the difference was significant (p < 0.001). Analyzing the relationship of Ki values to time-points, we observed a trend towards a decrease over time (r = - 0.61, p = 0.014). Both ADC values (r = - 0.58, p = 0.02) and ischemic lesion volumes (r = 0.75, p = 0.0015) correlated with Ki values. These results suggest that after ischemia-reperfusion in rats, BBB leakage is continuous during a 4-week period. Its magnitude diminishes over time and correlates with severity and extent of ischemic injury. © 2009 Elsevier Inc. All rights reserved.
dc.identifier.DOI-ID10.1016/j.expneurol.2009.06.002
dc.identifier.issn10902430
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18728
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectBlood-Brain Barrier
dc.subjectBrain Edema
dc.subjectBrain Ischemia
dc.subjectCerebrovascular Circulation
dc.subjectContrast Media
dc.subjectDisease Progression
dc.subjectGadolinium
dc.subjectImage Interpretation, Computer-Assisted
dc.subjectInfarction, Middle Cerebral Artery
dc.subjectLaser-Doppler Flowmetry
dc.subjectMagnetic Resonance Imaging
dc.subjectMale
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRecovery of Function
dc.subjectReperfusion Injury
dc.subjectTime Factors
dc.subjectgadolinium
dc.subjectanalytic method
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectarticle
dc.subjectblood brain barrier
dc.subjectbrain injury
dc.subjectbrain ischemia
dc.subjectcontrast enhancement
dc.subjectcontrolled study
dc.subjectdiffusion coefficient
dc.subjectdisease severity
dc.subjectflow rate
dc.subjectmale
dc.subjectmembrane permeability
dc.subjectnonhuman
dc.subjectnuclear magnetic resonance imaging
dc.subjectpriority journal
dc.subjectquantitative analysis
dc.subjectrat
dc.subjectreperfusion injury
dc.titlePost-ischemic leakiness of the blood-brain barrier: A quantitative and systematic assessment by Patlak plots
dc.typeArticle

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