Immunohistochemical staining of IGF-I, IGF-binding proteins-1 and -3, and transforming growth factor beta-3 in the umbilical cords of preeclamptic patients

Simple item page
dc.contributor.authorInan S.
dc.contributor.authorVatansever S.
dc.contributor.authorKuscu N.K.
dc.contributor.authorLaçin S.
dc.contributor.authorOzbilgin K.
dc.contributor.authorKoyuncu F.
dc.date.accessioned2024-07-22T08:25:13Z
dc.date.available2024-07-22T08:25:13Z
dc.date.issued2002
dc.description.abstractBackground. To detect the immunoreactivity of insulin-like growth factor-I, insulin-like growth factor-binding proteins-1 and -3 and transforming growth factor beta-3 in the umbilical cords of normal and preeclamptic patients. Methods. Umbilical cords were obtained from 15 normal and 15 preeclamptic patients. Immunoreactivities were determined using either indirect immunofluorescence or immunoperoxidase techniques on formalin-fixed, paraffin-embedded sections. Staining intensity was graded by a semiquantitative scoring method. The results were compared by Mann-Whitney U-test. Results. The umbilical cords were thinner and the vessels were hypoplastic in the preeclamptic group. Moderate staining intensity for insulin-like growth factor-I, insulin-like growth factor binding protein-1 and -3 and transforming growth factor-beta 3 was observed in normal patients. The preeclamptic group had mild and strong intensities for insulin-like growth factor-I and insulin-like growth factor binding protein-1, respectively, and intensity for insulin-like growth factor binding protein-3 did not change, but diffuse and increased intensity was observed for transforming growth factor-beta 3. Conclusion. Changes in the intensity of insulin-like growth factor-I and its major binding protein and the transformation of growth factor-beta 3 may play a role in the pathogenesis of preeclampsia by altering the structure and responsiveness of the umbilical cord.
dc.identifier.DOI-ID10.1034/j.1600-0412.2002.810815.x
dc.identifier.issn00016349
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20328
dc.language.isoEnglish
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAdult
dc.subjectCase-Control Studies
dc.subjectFemale
dc.subjectFormaldehyde
dc.subjectHumans
dc.subjectImmunoenzyme Techniques
dc.subjectImmunohistochemistry
dc.subjectInsulin-Like Growth Factor Binding Protein 1
dc.subjectInsulin-Like Growth Factor Binding Protein 3
dc.subjectInsulin-Like Growth Factor I
dc.subjectParaffin Embedding
dc.subjectPre-Eclampsia
dc.subjectPregnancy
dc.subjectStaining and Labeling
dc.subjectTransforming Growth Factor beta
dc.subjectTransforming Growth Factor beta3
dc.subjectUmbilical Cord
dc.subjectbinding protein
dc.subjectformaldehyde
dc.subjectparaffin
dc.subjectsomatomedin binding protein 1
dc.subjectsomatomedin binding protein 3
dc.subjectsomatomedin C
dc.subjecttransforming growth factor beta3
dc.subjectarticle
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman tissue
dc.subjecthypoplasia
dc.subjectimmunofluorescence
dc.subjectimmunohistochemistry
dc.subjectimmunoperoxidase staining
dc.subjectimmunoreactivity
dc.subjectintermethod comparison
dc.subjectpathogenesis
dc.subjectpreeclampsia
dc.subjectpriority journal
dc.subjectprotein binding
dc.subjectumbilical cord
dc.titleImmunohistochemical staining of IGF-I, IGF-binding proteins-1 and -3, and transforming growth factor beta-3 in the umbilical cords of preeclamptic patients
dc.typeArticle

Files

Collections

Scopus Koleksiyonu