Production, purification and characterization of a proteolytic enzyme from Streptomyces sp. 2M21

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dc.contributor.authorDeniz I.
dc.contributor.authorZihnioglu F.
dc.contributor.authorÖncel S.S.
dc.contributor.authorHames E.E.
dc.contributor.authorVardar-Sukan F.
dc.date.accessioned2024-07-22T08:08:36Z
dc.date.available2024-07-22T08:08:36Z
dc.date.issued2019
dc.description.abstractThe recent application studies on keratinase lead to new application areas for the enzyme to be used in novel industries such as pharmaceuticals and biodegradable composites. Thus, designing an economical and environmentally friendly keratinase production and purification with new features turned into an intriguing objective for its commercialization. In this study, keratinase enzyme was produced using a bioreactor of 5 L by a native Streptomyces sp. 2M21 and purified by ion exchange chromatography using step-wise gradient with 24.1-fold purification. Purified keratinase showed optimum activity at pH 9 and 30 °C. The enzyme was shown to be able to decompose different complex substrates such as chicken feather, wool and nail. Moreover, the addition of DTT enhanced the hydrolysis of wool and chicken feather to 3.4- and 3.2-fold, respectively. The potential of Streptomyces sp. to produce significant levels of keratinase using feathers as a substrate might establish cleaner applications for the production of high value-added products and degrading of keratin-containing wastes. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
dc.identifier.DOI-ID10.1080/10242422.2019.1568415
dc.identifier.issn10242422
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14469
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAnimals
dc.subjectBacteria
dc.subjectBioreactors
dc.subjectChromatographic analysis
dc.subjectEnzymes
dc.subjectIon chromatography
dc.subjectIon exchange
dc.subjectPurification
dc.subjectWool
dc.subjectYarn
dc.subjectfungal enzyme
dc.subjectkeratinase
dc.subjectmetal ion
dc.subjectproteinase
dc.subjectApplication studies
dc.subjectBiodegradable composites
dc.subjectChicken feathers
dc.subjectIon exchange chromatography
dc.subjectKeratinase
dc.subjectKeratinase productions
dc.subjectStreptomycessp
dc.subjectValue added products
dc.subjectArticle
dc.subjectchicken
dc.subjectenzyme activity
dc.subjectenzyme kinetics
dc.subjectenzyme purification
dc.subjectenzyme stability
dc.subjectenzyme synthesis
dc.subjectfeather
dc.subjectfungus culture
dc.subjecthydrolysis
dc.subjection exchange chromatography
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectpH
dc.subjectpolyacrylamide gel electrophoresis
dc.subjectprotein degradation
dc.subjectStreptomyces
dc.subjectStreptomyces sp. 2m21
dc.subjectSubstrates
dc.titleProduction, purification and characterization of a proteolytic enzyme from Streptomyces sp. 2M21
dc.typeArticle

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