Production of laccase from Trametes trogii TEM H2: A newly isolated white-rot fungus by air sampling

Simple item page
dc.contributor.authorKocyigit A.
dc.contributor.authorPazarbasi M.B.
dc.contributor.authorYasa I.
dc.contributor.authorOzdemir G.
dc.contributor.authorKaraboz I.
dc.date.accessioned2024-07-22T08:19:24Z
dc.date.available2024-07-22T08:19:24Z
dc.date.issued2012
dc.description.abstractThis work represents the first report of isolation of potential laccase producers by air sampling using media supplemented with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol for laccase production and secretion indicators. Nine fungal isolates showed positive reactions with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) and guaiacol. The isolate named TEM H2 exhibited the largest and intensive oxidation zones with 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate) (85 mm) and guaiacol (66 mm) and therefore it was selected for detailed investigations. The strain was identified as Trametes trogii TEM H2 due to the morphological characteristics and the comparison of internal transcribed spacer ribosomal DNA gene sequences. The laccase production was screened in different liquid cultures. The best laccase production medium was determined as soluble starch yeast extract medium in which laccase production was reached to a maximum level (989.6 U l-1) on the 8th day of cultivation. Effects of different initial pH values on laccase production were tested. Optimum pH value for laccase production in soluble starch yeast extract medium was determined as pH 3.0 with 15425.0 U l-1laccase production at 12th day of cultivation. In addition, effects of eight inducers (veratryl alcohol, ferulic acid, 1-Hydroxybenzotriazole, syringic acid, 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonate), 1 mmol l-1 CuSO4, 3% ethanol, guaiacol) were examined. Only cultures with 2,5-xylidine exhibited 1.9 fold increase in laccase activity reaching to 28890.0 U l-1. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.identifier.DOI-ID10.1002/jobm.201100341
dc.identifier.issn15214028
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17668
dc.language.isoEnglish
dc.subjectAir
dc.subjectAir Microbiology
dc.subjectDNA, Ribosomal Spacer
dc.subjectFungal Proteins
dc.subjectHydrogen-Ion Concentration
dc.subjectLaccase
dc.subjectOxidation-Reduction
dc.subjectPhylogeny
dc.subjectTrametes
dc.subjectFunalia trogii
dc.subjectFungi
dc.subjectTrametes
dc.subjectfungal protein
dc.subjectlaccase
dc.subjectribosomal spacer DNA
dc.subjectair
dc.subjectarticle
dc.subjectbiosynthesis
dc.subjectenzymology
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectoxidation reduction reaction
dc.subjectpH
dc.subjectphylogeny
dc.subjectTrametes
dc.titleProduction of laccase from Trametes trogii TEM H2: A newly isolated white-rot fungus by air sampling
dc.typeArticle

Files

Collections

Scopus Koleksiyonu