Cyclophosphamide suppresses spermatogenesis in the testis of mice through downregulation of miR-34b and miR-34c

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dc.contributor.authorÖzbilgin M.K.
dc.contributor.authorDemirören S.
dc.contributor.authorÜçöz M.
dc.contributor.authorOztatlici M.
dc.date.accessioned2024-07-22T08:05:49Z
dc.date.available2024-07-22T08:05:49Z
dc.date.issued2021
dc.description.abstractCyclophosphamide (CP) is commonly used as an anticancer agent but has been associated with high toxicity in several organs, including the testes. In this study, we aimed to evaluate the effects of CP-induced testicular toxicity, using glial cell line-derived neurotrophic factor (GDNF), occludin and transforming growth factor beta 3 (TGF-β3) primary antibodies, and miR-34b and miR-34c expressions. Eighteen young Balb/c male mice were divided into three groups. The control group received no treatment. The mice of CP group were injected 100 mg kg-1 day-1 CP for 5 days, and the same amount of saline was injected in the sham group. The animals were sacrificed 24 hr after the last injection. Immunohistochemical analysis of testicular tissues showed a decrease in both spermatogenic germ cell count and also GDNF, occludin expressions, but an increase in TGF-β3 expression in the CP group compared to the others group. The expressions of miR-34b and miR-34c were examined by qPCR technique, a significant decrease was observed in tissue samples in the CP-treated group. The expression of GDNF, occludin and TGF-β3 plays an important role in testicular injury caused by CP, and the decrease in the expression of miR-34b/c in tissue samples may be an important marker for the detection of testicular damage. © 2021 Wiley-VCH GmbH
dc.identifier.DOI-ID10.1111/and.14071
dc.identifier.issn03034569
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13269
dc.language.isoEnglish
dc.publisherJohn Wiley and Sons Inc
dc.rightsAll Open Access; Gold Open Access
dc.subjectAnimals
dc.subjectCyclophosphamide
dc.subjectDown-Regulation
dc.subjectMale
dc.subjectMice
dc.subjectMicroRNAs
dc.subjectSpermatogenesis
dc.subjectTestis
dc.subjectcomplementary DNA
dc.subjectcyclophosphamide
dc.subjectglial cell line derived neurotrophic factor
dc.subjectmicroRNA
dc.subjectmicroRNA 16
dc.subjectmicroRNA 192
dc.subjectmicroRNA 34b
dc.subjectmicroRNA 34c
dc.subjectneurotrophic factor
dc.subjectoccludin
dc.subjectsodium chloride
dc.subjecttransforming growth factor beta3
dc.subjectunclassified drug
dc.subjectcyclophosphamide
dc.subjectmicroRNA
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectArticle
dc.subjectazoospermia
dc.subjectcell count
dc.subjectcell degeneration
dc.subjectchemotherapy
dc.subjectcontrolled study
dc.subjectdesquamation
dc.subjectDNA synthesis
dc.subjectdown regulation
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectLeydig cell
dc.subjectmale
dc.subjectmale fertility
dc.subjectmouse
dc.subjectnonhuman
dc.subjectprotein determination
dc.subjectprotein expression
dc.subjectprotein expression level
dc.subjectprotein function
dc.subjectreal time polymerase chain reaction
dc.subjectseminiferous tubule
dc.subjectseminiferous tubule epithelium
dc.subjectSertoli cell
dc.subjectspermatocyte
dc.subjectspermatogenesis
dc.subjectspermatogonium
dc.subjecttestis
dc.subjecttestis biopsy
dc.subjecttestis injury
dc.subjecttestis tissue
dc.subjectTUNEL assay
dc.subjectanimal
dc.subjectdown regulation
dc.subjectgenetics
dc.subjectspermatogenesis
dc.titleCyclophosphamide suppresses spermatogenesis in the testis of mice through downregulation of miR-34b and miR-34c
dc.typeArticle

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