Pretreatment with pro- and synbiotics reduces peritonitis-induced acute lung injury in rats

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dc.contributor.authorTok D.
dc.contributor.authorIlkgul O.
dc.contributor.authorBengmark S.
dc.contributor.authorAydede H.
dc.contributor.authorErhan Y.
dc.contributor.authorTaneli F.
dc.contributor.authorUlman C.
dc.contributor.authorVatansever S.
dc.contributor.authorKose C.
dc.contributor.authorOk G.
dc.date.accessioned2024-07-22T08:23:11Z
dc.date.available2024-07-22T08:23:11Z
dc.date.issued2007
dc.description.abstractBACKGROUND: To study whether enteral pretreatment with a synbiotic composition of lactic acid bacteria and bioactive fibers can reduce peritonitis-induced lung neutrophil infiltration and tissue injury in rats. MATERIALS AND METHODS: Rats were divided into five groups, and subjected to induction of peritonitis-induced lung injury using a cecal ligation and puncture model (CLP). All animals were pretreated for 3 weeks prior the CLP by daily gavage with either (1) a synbiotic composition (10 CFU of Pediococcus pentosaceus 5-33:3, 10 CFU of Leuconostoc mesenteroides 77:1, 10 CFU of L. paracasei subspecies paracasei, 10 CFU of L. plantarum 2362 plus fermentable fibers), (2) fermentable fibers alone, (3) nonfermentable fibers, (4) a probiotic composition (10 CFU of P. pentosaceus 5-33:3, 10 CFU of L. mesenteroides 77:1, 10 CFU of L. paracasei subsp. paracasei, 10 CFU of L. plantarum 2,362), or (5) a heat-killed probiotic composition. All animals were killed 24 hours after CLP and lung tissue samples were studied for degree of neutrophil infiltration and levels of tumor necrosis factor (TNF)-α, Interleukin (IL)-1β. In addition the lung wet-to-dry tissue weight ratio, the myeloperoxidase activity, and malondialdehyde content were also assessed. RESULTS: No mortality was encountered in any of the groups. Histologic signs of lung injury (number of neutrophils and TNF-α, IL-1β staining) were observed in all groups except the synbiotic and probiotic treated groups. Myeloperoxidase activity and malondialdehyde content were significantly lower in the two lactobacillus- pretreated groups, with no difference between them. Heavy infiltration of lung tissue with neutrophils was observed only in fiber-treated (302.20 ± 7.92) and placebo-treated (266.90 ± 8.92) animals. This was totally abolished in the synbiotic-treated group (34.40 ± 2.49). Lung edema (wet-to-dry lung weight ratio) was significantly reduced in the synbiotic-treated group (4.92 ± 0.13 vs. 5.07 ± 0.08 and 5.39 ± 0.10, respectively). CONCLUSION: Three weeks of preoperative enteral administration of a synbiotic composition reduced peritonitis-induced acute lung injury in rats in a CLP model. © 2007 Lippincott Williams & Wilkins, Inc.
dc.identifier.DOI-ID10.1097/01.ta.0000236019.00650.00
dc.identifier.issn00225282
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19425
dc.language.isoEnglish
dc.publisherLippincott Williams and Wilkins
dc.subjectinterleukin 1beta
dc.subjectmalonaldehyde
dc.subjectmyeloperoxidase
dc.subjectprobiotic agent
dc.subjecttumor necrosis factor alpha
dc.subjectacute lung injury
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbody weight
dc.subjectcontrolled study
dc.subjectdrug activity
dc.subjectenzyme activity
dc.subjectfeeding
dc.subjectfemale
dc.subjectfermentation
dc.subjectlactic acid bacterium
dc.subjectLactobacillus
dc.subjectlung
dc.subjectlung edema
dc.subjectlung parenchyma
dc.subjectneutrophil chemotaxis
dc.subjectnonhuman
dc.subjectPediococcus
dc.subjectperitonitis
dc.subjectpriority journal
dc.subjectrat
dc.titlePretreatment with pro- and synbiotics reduces peritonitis-induced acute lung injury in rats
dc.typeArticle

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