The effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite

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dc.contributor.authorDeliloglu-Gurhan I.
dc.contributor.authorTuglu I.
dc.contributor.authorVatansever H.S.
dc.contributor.authorÖzdal-Kurt F.
dc.contributor.authorEkren H.
dc.contributor.authorTaylan M.
dc.contributor.authorSen B.H.
dc.date.accessioned2024-07-22T08:23:27Z
dc.date.available2024-07-22T08:23:27Z
dc.date.issued2006
dc.description.abstractObjective: To investigate the adhesive properties of bone marrow stromal cell (BMSC) on the hydroxyapatite (HA) particles and analyze their behavior. Methods: The study took place in the Department of the Histology and Embryology, Celal Bayar University, Manisa and in the Department of Bioengineering, Ege University, Izmir, Turkey between 2004 and 2005. We cultured BMSC from the mature rat tibia and differentiated to the osteoblasts by osteogenic medium. The BMSCs were subcultured and were taken to the HA substrate. We measured their proliferation capacity and viability with MTT assay using the spectrophotometric method. Furthermore, we identified the osteoblast-like cells by inummohistochemical staining of osteonectin and osteocalcin and we analyzed the behavior of the cells on different sized HA particles by SEM at the end of 3 days incubation. Results: Osteogenic medium caused the proliferation capacity of BMSC to speed up and the effects appeared earlier. We confirmed the osteoblastic differentiation by staining of most cells with osteoblastic markers. Subcultured cells were similarly adhesive to the HA particles and the osteogenic medium did not alter this behavior. They spread on the substrate similarly. Most of the cells demonstrated the cytoplasmic protrusion. Morphology of the cells did not change much with or without osteogenic medium. Different sizes of HA particles did not affect the adhesive properties of these cells except HA gel. The spreading and attachment ratios of the cells on HA gel were more than the others. Conclusion: We found that there was heterogeneity in BMSC on differentiation capacity to the osteoblast, which was a sign of a subpopulation. Adhesive cells showed similar morphology and behavior under the effect of osteogenic medium. The only difference was the spreading capacity on the HA gel where cell used this substrate more effectively for adhesion.
dc.identifier.issn03795284
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19551
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectBiocompatible Materials
dc.subjectBone Marrow Cells
dc.subjectCell Adhesion
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCells, Cultured
dc.subjectDurapatite
dc.subjectMale
dc.subjectOsteogenesis
dc.subjectRats
dc.subjectRats, Wistar
dc.subjecthydroxyapatite
dc.subjectosteocalcin
dc.subjectosteonectin
dc.subjectanimal cell
dc.subjectarticle
dc.subjectbone development
dc.subjectbone marrow cell
dc.subjectbone mineralization
dc.subjectcell adhesion
dc.subjectcell differentiation
dc.subjectcell function
dc.subjectcell maturation
dc.subjectcell proliferation
dc.subjectcell structure
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectculture medium
dc.subjectimmunocytochemistry
dc.subjectimmunohistochemistry
dc.subjectincubation time
dc.subjectnonhuman
dc.subjectosteoblast
dc.subjectrat
dc.subjectscanning electron microscopy
dc.subjectspectrophotometry
dc.titleThe effect of osteogenic medium on the adhesion of rat bone marrow stromal cell to the hydroxyapatite
dc.typeArticle

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