Keratinocytes derived from embryonic stem cells induce wound healing in mice

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dc.contributor.authorUluer E.T.
dc.contributor.authorVatansever H.S.
dc.contributor.authorAydede H.
dc.contributor.authorOzbilgin M.K.
dc.date.accessioned2024-07-22T08:08:49Z
dc.date.available2024-07-22T08:08:49Z
dc.date.issued2019
dc.description.abstractThe skin plays an important role in defending the body against the environment. Treatments for burns and skin injuries that use autologous or allogenic skin grafts derived from adult or embryonic stem cells are promising. Embryonic stem cells are candidates for regenerative and reparative medicine. We investigated the utility of keratinocyte-like cells, which are differentiated from mouse embryonic stem cells, for wound healing using a mouse surgical wound model. Mice were allocated to the following groups: experimental, in which dressing and differentiated cells were applied after the surgical wound was created; control, in which only the surgical wound was created; sham, in which only the dressing was applied after the surgical wound was created; and untreated animal controls with healthy skin. Biopsies were taken from each group on days 3, 5 and 7 after cell transfer. Samples were fixed in formalin, then stained with Masson’s trichrome and primary antibodies to interleukin-8 (IL-8), fibroblast growth factor-2 (FGF-2), monocyte chemoattractant protein-1 (MCP-1), collagen-1 and epidermal growth factor (EGF) using the indirect immunoperoxidase technique for light microscopy. Wound healing was faster in the experimental group compared to the sham and control groups. The experimental group exhibited increased expression of IL-8, FGF-2 and MCP-1 during early stages of wound healing (inflammation) and collagen-1 and EGF expression during late stages of wound healing (proliferation and remodeling). Keratinocytes derived from embryonic stem cells improved wound healing and influenced the wound healing stages. © 2018, © 2018 The Biological Stain Commission.
dc.identifier.DOI-ID10.1080/10520295.2018.1541479
dc.identifier.issn10520295
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14548
dc.language.isoEnglish
dc.publisherTaylor and Francis Ltd
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectChemokine CCL2
dc.subjectCollagen
dc.subjectEmbryonic Stem Cells
dc.subjectEpidermal Growth Factor
dc.subjectFibroblast Growth Factor 2
dc.subjectInterleukin-8
dc.subjectKeratinocytes
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectWound Healing
dc.subjectCcl2 protein, mouse
dc.subjectcollagen
dc.subjectepidermal growth factor
dc.subjectfibroblast growth factor 2
dc.subjectinterleukin 8
dc.subjectmonocyte chemotactic protein 1
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectcell differentiation
dc.subjectembryonic stem cell
dc.subjectgenetics
dc.subjectkeratinocyte
dc.subjectmale
dc.subjectmetabolism
dc.subjectmouse
dc.subjectphysiology
dc.subjectwound healing
dc.titleKeratinocytes derived from embryonic stem cells induce wound healing in mice
dc.typeArticle

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