Role of VPAC1 and VPAC2 receptors in the etiology of pregnancy rhinitis: an experimental study in rats

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dc.contributor.authorUlkumen B.
dc.contributor.authorBatir M.B.
dc.contributor.authorArtunc Ulkumen B.
dc.contributor.authorPala H.G.
dc.contributor.authorVatansever S.
dc.contributor.authorCam S.
dc.date.accessioned2024-07-22T08:04:18Z
dc.date.available2024-07-22T08:04:18Z
dc.date.issued2022
dc.description.abstractIntroduction: Pregnancy rhinitis is a common sex hormone-related otorhinolaryngological disorder. There are some epidemiological and physiological studies on pregnancy rhinitis, but histopathological and biomolecular changes have not been studied thoroughly. Objectives: The receptors VPAC1 and VPAC2 are known for their roles in allergic rhinitis. On the other hand, activation of subclinical allergy has been suggested in the pathophysiology of pregnancy rhinitis. Therefore, we aimed to compare the physiological and gestational pattern of VPAC1 and VPAC2 expression in rat nasal mucosa. Methods: Twenty adult Wister albino female rats were enrolled into the study. Two groups constituted as 10 control (group A) and 10 pregnant (group B) rats. They were fed ad libitum and sheltered at room temperature (22°±2 °C). The rats were sacrificed at the 20th day of gestation by intraperitoneal injection of 400 mg/kg Na-pentobarbitone. Then, 10 − 15 mL of blood was taken, and samples were reserved for the detection of serum estradiol and progesterone levels by ELISA test. The nasal septum was resected and divided in half for immunohistochemical analyses and real time polymerase chain reaction testing of VPAC1 and VPAC2. Results: VPAC1 and VPAC2 were found to be in all layers of septal specimens, but the immunostaining of surface epithelium was more distinct in specimens of both groups. We demonstrated higher overall staining intensity in the pregnant group. PCR revealed significant increase in expression of VPAC1 (p = 0.023) and VPAC2 (p = 0.021) in pregnant group when compared with control group. In addition, we demonstrated upregulatory effect of estradiol and progesterone on the vasoactive intestinal peptide receptor expression. Conclusions: Gestational up-regulation of nasal VPAC1 and VPAC2 was shown both by PCR and immunohistochemical analysis. These findings support the hypothesis that PR is caused by the activation of subclinical allergy that is present before pregnancy. © 2020 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial
dc.identifier.DOI-ID10.1016/j.bjorl.2020.06.015
dc.identifier.issn18088694
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12653
dc.language.isoEnglish
dc.publisherElsevier Editora Ltda
dc.rightsAll Open Access; Gold Open Access; Green Open Access
dc.subjectAnimals
dc.subjectEstradiol
dc.subjectFemale
dc.subjectHypersensitivity
dc.subjectPregnancy
dc.subjectProgesterone
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectRhinitis
dc.subjectestradiol
dc.subjectpentobarbital
dc.subjectprogesterone
dc.subjectvasoactive intestinal polypeptide receptor 1
dc.subjectvasoactive intestinal polypeptide receptor 2
dc.subjectestradiol
dc.subjectprogesterone
dc.subjectalbino rat
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbasement membrane
dc.subjectblood sampling
dc.subjectcontrolled study
dc.subjectenzyme linked immunosorbent assay
dc.subjectestradiol blood level
dc.subjectexperimental study
dc.subjectfemale
dc.subjectgestational age
dc.subjectimmunohistochemistry
dc.subjectlamina propria
dc.subjectnonhuman
dc.subjectnose cartilage
dc.subjectnose epithelium
dc.subjectnose mucosa
dc.subjectnose septum
dc.subjectpregnancy rhinitis
dc.subjectprogesterone blood level
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectrat
dc.subjectreal time polymerase chain reaction
dc.subjectrhinitis
dc.subjectroom temperature
dc.subjectanimal
dc.subjecthypersensitivity
dc.subjectpregnancy
dc.subjectWistar rat
dc.titleRole of VPAC1 and VPAC2 receptors in the etiology of pregnancy rhinitis: an experimental study in rats
dc.typeArticle

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