Antibiotic treatment is superior to ursodeoxycholic acid on total parenteral nutrition associated hepatic dysfunction

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dc.contributor.authorGünşar C.
dc.contributor.authorVatansever S.
dc.contributor.authorVar A.
dc.contributor.authorAygören R.
dc.contributor.authorYilmaz O.
dc.contributor.authorTürköz E.
dc.contributor.authorŞencan A.
dc.contributor.authorMir E.
dc.date.accessioned2024-07-22T08:20:58Z
dc.date.available2024-07-22T08:20:58Z
dc.date.issued2010
dc.description.abstractPurpose This study aimed to investigate the apoptotic mechanisms, oxidative stress, and mechanisms of effect of antibiotics and ursodeoxycholic acid (UDCA) in total parenteral nutrition (TPN)-associated liver injury. Methods Four groups of young rabbits were used in the study as follows: Group 1 (n: 7): TPN + Metronidazole (30 mg/kg IV) + Gentamicin (6 mg/kg IV); Group 2 (n: 7): TPN + UDCA (15 mg/kg per oral); Group 3 (n: 6): TPN only; and Group 4 (n: 7): Control group. After 10 days, the animals were killed and livers were removed. Hepatic apoptosis, apoptotic proteins, malondialdehyde (MDA) and myeloperoxidase (MPO) levels were studied in liver, and direct bilirubin values were assessed in the blood samples. Results Direct bilirubin increased with TPN, and antibiotic combination, as the most effective group, significantly lowered its levels (p<0.01). MDA values also showed significant differences in comparisons between G1 and G3 (p<0.05) and G1-4 (p<0.01). An increased number of apoptotic cells was detected particularly in G2 and G3, whereas the lowest levels, other than in the control group, were found in G1. All TUNEL-positive cell number data were statistically significant except between G2 and G3(p<0.05). Caspase-3 and Bax immunoreactivities were greatest in G2. Significant differences were shown in caspase-3 immunoreactivity between the groups (p<0.01), except between G1 and G3 (p>0.05). All comparisons between the groups were significant for Bax (p<0.01). In contrast, Bcl-2 immunoreactivity was moderate and highest in G1: comparisons between G1 and the other groups demonstrated statistically significant differences (p<0.01). Fas-L immunoreactivity was greatest in G2, and all comparisons between the groups were statistically significant (p<0.01). Conclusions Metronidazole and gentamicin combination is effective on TPN-induced liver injury by the Bcl-2 antiapoptotic pathway, total anti-apoptotic effect and by decreasing bilirubin levels. Oxidative injury in the liver increased with therapy. UDCA seems less effective on TPN-associated liver injury. © Springer-Verlag 2010.
dc.identifier.DOI-ID10.1007/s00383-010-2578-5
dc.identifier.issn01790358
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18394
dc.language.isoEnglish
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectbcl-2-Associated X Protein
dc.subjectBilirubin
dc.subjectCaspase 3
dc.subjectFas Ligand Protein
dc.subjectGentamicins
dc.subjectImmunoenzyme Techniques
dc.subjectIn Situ Nick-End Labeling
dc.subjectLiver Diseases
dc.subjectMalondialdehyde
dc.subjectMetronidazole
dc.subjectOxidative Stress
dc.subjectParenteral Nutrition, Total
dc.subjectPeroxidase
dc.subjectRabbits
dc.subjectUrsodeoxycholic Acid
dc.subjectbilirubin
dc.subjectcaspase 3
dc.subjectFas ligand
dc.subjectgentamicin
dc.subjectmalonaldehyde
dc.subjectmetronidazole
dc.subjectmyeloperoxidase
dc.subjectprotein Bax
dc.subjectprotein bcl 2
dc.subjectursodeoxycholic acid
dc.subjectbilirubin
dc.subjectcaspase 3
dc.subjectFas ligand
dc.subjectgentamicin
dc.subjectmalonaldehyde
dc.subjectmetronidazole
dc.subjectperoxidase
dc.subjectprotein Bax
dc.subjectursodeoxycholic acid
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantibiotic therapy
dc.subjectapoptosis
dc.subjectarticle
dc.subjectbilirubin blood level
dc.subjectcell count
dc.subjectcontrolled study
dc.subjectdrug efficacy
dc.subjectdrug mechanism
dc.subjectimmunoreactivity
dc.subjectliver injury
dc.subjectnick end labeling
dc.subjectnonhuman
dc.subjectoxidative stress
dc.subjectpriority journal
dc.subjectrabbit
dc.subjecttotal parenteral nutrition
dc.subjecttreatment outcome
dc.subjectanalysis of variance
dc.subjectanimal
dc.subjectblood
dc.subjectenzyme immunoassay
dc.subjectliver disease
dc.subjectmetabolism
dc.subjecttotal parenteral nutrition
dc.titleAntibiotic treatment is superior to ursodeoxycholic acid on total parenteral nutrition associated hepatic dysfunction
dc.typeArticle

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