Fatty acid composition and anticancer activity in colon carcinoma cell lines of Prunus dulcis seed oil
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Date
2017
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Abstract
Context: Almond oil is used in traditional and complementary therapies for its numerous health benefits due to high unsaturated fatty acids content. Objectives: This study investigated the composition and in vitro anticancer activity of almond oil from Northern Cyprus and compared with almond oil from Turkey. Materials and methods: Almond oil from Northern Cyprus was obtained by supercritical CO2 extraction and analyzed by GC-MS. Almond oil of Turkey was provided from Turkish pharmacies. Different concentrations of almond oils were incubated for 24 and 48 h with Colo-320 and Colo-741 cells. Cell growth and cytotoxicity were measured by MTT assays. Anticancer and antiprolifetarive activities of almond oils were investigated by immunocytochemistry using antibodies directed against to BMP-2, β-catenin, Ki-67, LGR-5 and Jagged 1. Results: Oleic acid (77.8%; 75.3%), linoleic acid (13.5%; 15.8%), palmitic acid (7.4%; 6.3%), were determined as the major compounds of almond oil from Northern Cyprus and Turkey, respectively. In the MTT assay, both almond oils were found to be active against Colo-320 and Colo-741 cells with 1:1 dilution for both 24 h and 48 h. As a result of immunohistochemical staining, while both almond oils exhibited significant antiproliferative and anticancer activity, these activities were more similar in Colo-320 cells which were treated with Northern Cyprus almond oil. Discussion and conclusion: Almond oil from Northern Cyprus and Turkey may have anticancer and antiproliferative effects on colon cancer cells through molecular signalling pathways and, thus, they could be potential novel therapeutic agents. © 2017 The Author(s).
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Antineoplastic Agents, Phytogenic , Cell Line, Tumor , Cell Survival , Colonic Neoplasms , Dose-Response Relationship, Drug , Fatty Acids , Humans , Plant Oils , Prunus dulcis , Seeds , almond oil , beta catenin , bone morphogenetic protein 2 , guanine nucleotide binding protein , Ki 67 antigen , leucine rich repeat containing G protein coupled receptor 5 , linoleic acid , oleic acid , palmitic acid , protein Jagged 1 , unclassified drug , almond oil , antineoplastic agent , fatty acid , vegetable oil , almond , antineoplastic activity , antiproliferative activity , Article , cell growth , COLO 320 cell line , colon carcinoma , colon carcinoma cell line , controlled study , Cyprus , cytotoxicity , human , human cell , immunocytochemistry , in vitro study , lipid composition , MTT assay , oilseed , Turkey (republic) , almond , cell survival , Colonic Neoplasms , dose response , drug effects , isolation and purification , pathology , physiology , plant seed , tumor cell line