First isolation of a trh positive V. alginolyticus from Atlantic bluefin tuna (Thunnus thynnus) farmed in Turkey

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dc.contributor.authorAvsever M.L.
dc.date.accessioned2024-07-22T08:04:59Z
dc.date.available2024-07-22T08:04:59Z
dc.date.issued2022
dc.description.abstractPopularity of sushi-sashimi (raw-almost raw fish) has increased around the globe, and Atlantic bluefin tuna is among the most frequently used fish in these dishes. In this study, we used two adult Atlantic bluefin tunas sent to the laboratory from a farm in the Aegean region of Turkey in 2014. We isolated trh (Thermostable direct hemolysin-related hemolysin gene) positive Vibrio alginolyticus from the internal organs of these two tunas. Isolates were obtained by conventional microbiological methods and identified with Vitek 2 Compact fully automatic identification device. Confirmation of isolates and investigation of thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) genes were carried out by a Polymerase Chain Reaction (PCR) method. Two V.alginolyticus isolates from these two tuna samples were found to be trh positive and tdh negative. The aim of this study is to report first isolation of a trh positive V. alginolyticus from two bluefin tunas farmed in the Aegean sea, to draw attention to possible dangers in raw-almost raw fish consumption and supply information for further epidemiological studies. However, more samples are necessary for elucidating the prevalence of high virulence food pathogens in tuna. However, due to their economic value, multiple tuna samples are rarely sent to laboratories for diagnostic purposes. © 2022, Meriç Lutfi Avsever
dc.identifier.DOI-ID10.12681/jhvms.25118
dc.identifier.issn17922720
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12942
dc.language.isoEnglish
dc.publisherHellenic Veterinary Medical Society
dc.rightsAll Open Access; Hybrid Gold Open Access
dc.subjectflorfenicol
dc.subjecthemolysin
dc.subjectAegean Sea
dc.subjectArticle
dc.subjectbacterial colonization
dc.subjectbacterium isolation
dc.subjectDNA extraction
dc.subjectDNA fragmentation assay
dc.subjectfish consumption
dc.subjectisolation
dc.subjectnonhuman
dc.subjectpolymerase chain reaction
dc.subjectpublic health
dc.subjecttuna
dc.subjectVibrio alginolyticus
dc.subjectVibrio anguillarum
dc.subjectVibrio cholerae
dc.subjectVibrio parahaemolyticus
dc.subjectvibriosis
dc.titleFirst isolation of a trh positive V. alginolyticus from Atlantic bluefin tuna (Thunnus thynnus) farmed in Turkey
dc.typeArticle

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