The Role of TREK-1 and AQP5 in Gonadocorticoid-Related Voice Disorders

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dc.contributor.authorUlkumen B.
dc.contributor.authorArtunc Ulkumen B.
dc.contributor.authorBatir M.B.
dc.contributor.authorCam S.
dc.contributor.authorVatansever S.
dc.date.accessioned2024-07-22T08:04:40Z
dc.date.available2024-07-22T08:04:40Z
dc.date.issued2022
dc.description.abstractObjectives: TWIK-related potassium channel-1 (TREK-1) and Aquaporin 5 (AQP5) are involved in epithelial integrity and fluid transport, respectively. In this study, we aimed to compare physiological and gestational patterns of TREK-1 and AQP5 location and expression in rat larynx. Our secondary objective was to reveal the effect of estradiol (E2) and progesterone (PG) on these two biomolecules. Methods: This study was conducted on 20 Wister albino female rats which were assigned as control (group A) and pregnant group (group B). The rats were sacrificed at 20th day of pregnancy. Blood was obtained directly from the ventricle for detection of serum E2 and PG levels. Larynx was resected for immunohistochemical analyses and real-time polymerase chain reaction testing for detection of TREK-1 and AQP5 staining and expression, respectively. Results: Relative TREK-1 (P = 0.035) and AQP5 (P = 0.019) expression was found to be significantly high in group B when compared with group A. We found positive correlation between serum E2 levels and both biomolecules (TREK-1; P = 0.018, AQP5; P = 0.016). We also found positive correlation between serum PG levels and both biomolecules (TREK-1; P = 0.001, AQP5; P = 0.019). TREK-1 immunostaining was found to be higher in surface epithelium and lamina propria of vocal cord mucosa. AQP5 was particularly found to be located in basement membrane and adjacent superficial lamina propria. We revealed the physiological and gestational pattern of laryngeal TREK-1 and AQP5 expression for the first time. Gestational expression of both TREK-1 and AQP5 was found to be increased. Stimulatory effect of E2 and PG on laryngeal TREK-1 and AQP5 expression was also revealed. Conclusions: We revealed upregulatory effect of E2 and PG on laryngeal TREK-1 and AQP5 expression. Based on this finding, it can be suggested that TREK-1 and AQP5 play role in biomolecular processes leading gonadocorticoid-related voice changes. © 2020 The Voice Foundation
dc.identifier.DOI-ID10.1016/j.jvoice.2020.04.024
dc.identifier.issn08921997
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12776
dc.language.isoEnglish
dc.publisherElsevier Inc.
dc.subjectAnimals
dc.subjectAquaporin 5
dc.subjectFemale
dc.subjectHumans
dc.subjectPotassium Channels, Tandem Pore Domain
dc.subjectPregnancy
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectVoice Disorders
dc.subjectaquaporin 5
dc.subjectestradiol
dc.subjectpotassium channel
dc.subjectprogesterone
dc.subjectprotein twik related potassium channel 1
dc.subjectunclassified drug
dc.subjectAQP5 protein, human
dc.subjectAqp5 protein, rat
dc.subjectaquaporin 5
dc.subjectpotassium channel protein TREK-1
dc.subjecttandem pore domain potassium channel
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbasement membrane
dc.subjectcontrolled study
dc.subjectestradiol blood level
dc.subjectfemale
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectlamina propria
dc.subjectlarynx
dc.subjectmale
dc.subjectmRNA expression level
dc.subjectnonhuman
dc.subjectpregnancy
dc.subjectpreliminary data
dc.subjectprogesterone blood level
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectrat
dc.subjectreal time polymerase chain reaction
dc.subjectsquamous epithelium
dc.subjectupregulation
dc.subjectvocal cord
dc.subjectvoice
dc.subjectvoice disorder
dc.subjectWistar rat
dc.subjectanimal
dc.subjectgenetics
dc.subjecthuman
dc.subjectmetabolism
dc.titleThe Role of TREK-1 and AQP5 in Gonadocorticoid-Related Voice Disorders
dc.typeArticle

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