Neurotoxic effect of statins on mouse neuroblastoma NB2a cell line

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dc.contributor.authorVural K.
dc.contributor.authorTuglu M.I.
dc.date.accessioned2024-07-22T08:20:06Z
dc.date.available2024-07-22T08:20:06Z
dc.date.issued2011
dc.description.abstractObjective: Evidences from cell culture experiments suggest a link between cholesterol and nervous system disease. Statins may have neurotoxic or neuroprotective effects, but these effects remain controversial. Therefore, the present study was aimed to investigate the possible toxicity of statins on a neurite outgrowth in mouse neuroblastoma NB2a cell line. Materials and Methods: We have utilized d-cAMP-induced terminally differentiated NB2a cells in culture as an experimental model to study the effects of statins. The cell survival and proliferation were studied by MTT. Measurement of neurite outgrowth was done by neurotoxicity screening test. NB2a cell differentiation was achieved by serum free medium plus 0.5 mM dibutyryl cAMP. Cells were incubated for 24 hours at 37°C. After this period, lovastatin, mevastatin and atorvastatin were added to wells at different concentrations (1, 3, 10, 100 μM). Approximately 100 cells were chosen for each sample and examined randomly 24 hours later, from 10 different fields. Total length of neurite was photographed microscopically and measured by image analyze software. Changes in neurite lengths were expressed as % inhibition compared to that of the control group. Results: Results showed that three statins at high concentrations induced neurite inhibition, inhibited proliferation and reduced the viability of differentiated neuroblastoma NB2a cells. Conclusions: Our results suggest that statins could act as a neurotoxic agent at high doses depending upon their concentrations. These results require further investigation at ultra structural and molecular levels to understand long term side effects for clinical safety of statins.
dc.identifier.issn11283602
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17979
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectDose-Response Relationship, Drug
dc.subjectHeptanoic Acids
dc.subjectHydroxymethylglutaryl-CoA Reductase Inhibitors
dc.subjectLovastatin
dc.subjectMice
dc.subjectNeurites
dc.subjectNeuroblastoma
dc.subjectPyrroles
dc.subjectatorvastatin
dc.subjectcompactin
dc.subjectcyclic AMP
dc.subjectmevinolin
dc.subjectanimal cell
dc.subjectarticle
dc.subjectcell differentiation
dc.subjectcell proliferation
dc.subjectcell survival
dc.subjectcomputer program
dc.subjectconcentration response
dc.subjectimage analysis
dc.subjectmouse
dc.subjectnerve fiber growth
dc.subjectneuroblastoma
dc.subjectneurotoxicity
dc.subjectnonhuman
dc.titleNeurotoxic effect of statins on mouse neuroblastoma NB2a cell line
dc.typeArticle

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