Biological Response of Osteoblastic and Chondrogenic Cells to Graphene-Containing PCL/Bioactive Glass Bilayered Scaffolds for Osteochondral Tissue Engineering Applications

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dc.contributor.authorDeliormanlı A.M.
dc.contributor.authorAtmaca H.
dc.date.accessioned2024-07-22T08:09:29Z
dc.date.available2024-07-22T08:09:29Z
dc.date.issued2018
dc.description.abstractGraphene-containing 13-93 bioactive glass and poly(ε-caprolactone)-based bilayer, electrically conductive scaffolds were prepared for osteochondral tissue repair. Biological response of osteoblastic MC3T3-E1 and chondrogenic ATDC5 cells to the composite scaffolds was assessed under mono-culture and co-culture conditions. Cytotoxicity was investigated using MTT assay, cartilage matrix production was evaluated by Alcian blue staining, and mineralization of both types of cells in the different culture systems was observed by Alizarin red S staining. Results showed that osteoblastic and chondrogenic cells utilized in the study did not show toxic response to the prepared scaffolds under mono-culture conditions and higher cell viability rates were obtained in co-culture conditions. Larger mineralized areas were determined under co-culture conditions and calcium deposition amount significantly increased compared with that in control group samples after 21 days. Additionally, the amount of glycosaminoglycans synthesized in co-culture was higher compared to mono-culture conditions. Electric stimulation applied under mono-culture conditions suppressed the viability of MC3T3-E1 cells whereas it enhanced the viability rates of ATDC5 cells. The study suggests that the designed bilayered osteochondral constructs have the potential for osteochondral defect repair. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
dc.identifier.DOI-ID10.1007/s12010-018-2758-7
dc.identifier.issn02732289
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14843
dc.language.isoEnglish
dc.publisherHumana Press Inc.
dc.subjectAnimals
dc.subjectBone and Bones
dc.subjectCartilage
dc.subjectCell Line
dc.subjectCeramics
dc.subjectChondrocytes
dc.subjectElectric Stimulation
dc.subjectGraphite
dc.subjectMice
dc.subjectOsteoblasts
dc.subjectPolyesters
dc.subjectTissue Engineering
dc.subjectBioactive glass
dc.subjectCell engineering
dc.subjectCells
dc.subjectCytology
dc.subjectElectrophysiology
dc.subjectElectrotherapeutics
dc.subjectGraphene
dc.subjectRepair
dc.subjectTissue
dc.subjectalcian blue
dc.subjectalizarin red s
dc.subjectglass
dc.subjectglycosaminoglycan
dc.subjectgraphene
dc.subjectpolycaprolactone
dc.subjectBioglass
dc.subjectgraphite
dc.subjectpolycaprolactone
dc.subjectpolyester
dc.subjectCaprolactone
dc.subjectCo-cultures
dc.subjectElectrically conductive
dc.subjectOsteochondral
dc.subjectOsteochondral defects
dc.subjectOsteochondral tissue engineering
dc.subjectOsteochondral tissues
dc.subjectPoly (epsiloncaprolactone)
dc.subjectArticle
dc.subjectbilayer membrane
dc.subjectcartilage matrix
dc.subjectcell viability
dc.subjectchondrocyte
dc.subjectcoculture
dc.subjectelectrostimulation
dc.subjectMC3T3-E1 cell line
dc.subjectmonoculture
dc.subjectosteoblast
dc.subjecttissue engineering
dc.subjecttissue repair
dc.subjectanimal
dc.subjectbone
dc.subjectcartilage
dc.subjectcell line
dc.subjectceramics
dc.subjectchemistry
dc.subjectchondrocyte
dc.subjectcytology
dc.subjectmetabolism
dc.subjectmouse
dc.subjectosteoblast
dc.subjectprocedures
dc.subjecttissue engineering
dc.subjectScaffolds (biology)
dc.titleBiological Response of Osteoblastic and Chondrogenic Cells to Graphene-Containing PCL/Bioactive Glass Bilayered Scaffolds for Osteochondral Tissue Engineering Applications
dc.typeArticle

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