Vitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats

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dc.contributor.authorŞahin S.
dc.contributor.authorGürgen S.G.
dc.contributor.authorYazar U.
dc.contributor.authorİnce İ.
dc.contributor.authorKamaşak T.
dc.contributor.authorAcar Arslan E.
dc.contributor.authorDiler Durgut B.
dc.contributor.authorDilber B.
dc.contributor.authorCansu A.
dc.date.accessioned2024-07-22T08:09:11Z
dc.date.available2024-07-22T08:09:11Z
dc.date.issued2019
dc.description.abstractObjectives: The hippocampus is susceptible to damage in patients with epilepsy and in animals with seizures caused by excitotoxic agents. The effect of vitamin D on hippocampal apoptosis related with seizures has not been reported. However, epileptic patients have an increased risk of hypovitaminosis D which is most likely due to the effects of antiepileptic drugs. Therefore, in this study, it was aimed to evaluate the effects of vitamin D on hippocampal apoptosis related with seizures by using pentylenetetrazol (PTZ) and kainic acid (KA) in rats. Methods: Male Sprague Dawley rats, aged 5.5 weeks, were randomly divided into six groups: control, vitamin D, PTZ, KA, PTZ + vitamin D and KA + vitamin D groups. The groups that received vitamin D were given 500 IU/kg of vitamin D daily for two weeks in addition to a standard diet. At the end of this period, PTZ and KA were applied to trigger seizures in the rats in the seizure groups. 24 h after the administration of PTZ and KA, the rats were decapitated. In the hippocampal region, apoptosis was assessed by TUNEL and brain-derived neurotrophic factor (BDNF), Bax, caspase-3 and c-fos activation were evaluated by immunohistochemical method. Results: BDNF level increased while c-fos, Bax and caspase-3 levels decreased (p < 0.0001, in all) in the hippocampal neurons of the groups that were pre-treated with vitamin D before the administration of PTZ and KA, in comparison with the PTZ and KA groups. Vitamin D significantly decreased the number of apoptotic cells in these rats in comparison with the PTZ and KA groups (p < 0.0001). Conclusion: This study indicates that vitamin D has neuroprotective effects on hippocampal apoptosis induced by PTZ and KA in rats. With this study it is suggested that keeping vitamin D levels within normal limits may be beneficial for patients with epilepsy, especially children. © 2018 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.eplepsyres.2018.12.005
dc.identifier.issn09201211
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14710
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectbcl-2-Associated X Protein
dc.subjectBrain-Derived Neurotrophic Factor
dc.subjectCaspase 3
dc.subjectConvulsants
dc.subjectDisease Models, Animal
dc.subjectHippocampus
dc.subjectIn Situ Nick-End Labeling
dc.subjectKainic Acid
dc.subjectMale
dc.subjectNeurons
dc.subjectNeuroprotective Agents
dc.subjectPentylenetetrazole
dc.subjectProto-Oncogene Proteins c-fos
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectSeizures
dc.subjectVitamin D
dc.subjectbrain derived neurotrophic factor
dc.subjectcaspase 3
dc.subjectdevit 3
dc.subjectprotein Bax
dc.subjectvitamin D
dc.subjectbrain derived neurotrophic factor
dc.subjectcaspase 3
dc.subjectconvulsant agent
dc.subjectkainic acid
dc.subjectneuroprotective agent
dc.subjectpentetrazole
dc.subjectprotein Bax
dc.subjectprotein c fos
dc.subjectvitamin D
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectdrug efficacy
dc.subjectenzyme activation
dc.subjecthippocampal CA3 region
dc.subjecthippocampus
dc.subjectimmunohistochemistry
dc.subjectkainic acid-induced seizure
dc.subjectmale
dc.subjectneuroprotection
dc.subjectnonhuman
dc.subjectoutcome assessment
dc.subjectpentylenetetrazole-induced seizure
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrandomized controlled trial
dc.subjectrat
dc.subjectTUNEL assay
dc.subjectanimal
dc.subjectapoptosis
dc.subjectchemically induced
dc.subjectdisease model
dc.subjectdrug effect
dc.subjecthippocampus
dc.subjectmetabolism
dc.subjectnerve cell
dc.subjectpathology
dc.subjectseizure
dc.subjectSprague Dawley rat
dc.titleVitamin D protects against hippocampal apoptosis related with seizures induced by kainic acid and pentylenetetrazol in rats
dc.typeArticle

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