Effects of Thymus serpyllum extract on cell proliferation, apoptosis and epigenetic events in human breast cancer Cells

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dc.contributor.authorBozkurt E.
dc.contributor.authorAtmaca H.
dc.contributor.authorKisim A.
dc.contributor.authorUzunoglu S.
dc.contributor.authorUslu R.
dc.contributor.authorKaraca B.
dc.date.accessioned2024-07-22T08:19:08Z
dc.date.available2024-07-22T08:19:08Z
dc.date.issued2012
dc.description.abstractThymus (T.) serpyllum (wild thyme) is an aromatic medicinal plant due to its several biological properties, including anticancer activity. Breast cancer is one of the most common malignancies and increasing evidence supports that it is not only a genetic but also an epigenetic disease. Epigenetics investigates changes in gene expression caused by mechanisms that do not involve alterations in DNA sequence. DNA methylation and histone acetylation are the most widely studied epigenetic changes in cancer cells. This study evaluated the effects of T. serpyllum on apoptosis and epigenetic events in breast cancer cells. XTT cell viability assay was used to determine cytotoxicity. DNA fragmentation and caspase 3/7 activity assays were used in the assesment of apoptosis. DNA methyltransferase (DNMT) and histone deacetylase (HDAC) activities were evaluated by ELISA and verified by qRT-PCR. T. serpyllum extract induced significant cytotoxicity in breast cancer cells (MCF-7 and MDAMB-231) but not in normal cells. It also induced apoptosis and inhibited the DNMT and HDAC activities in MDA-MB-231 cells. In the present study, the first preliminary data on the effects of the methanolic extract of T. serpyllum in normal and breast cancer cells were obtained and suggest that T. serpyllum may be a promising candidate in the development of novel therapeutic drugs for breast cancer treatment. Copyright © 2012, Taylor & Francis Group, LLC.
dc.identifier.DOI-ID10.1080/01635581.2012.719658
dc.identifier.issn15327914
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17546
dc.language.isoEnglish
dc.subjectAntineoplastic Agents, Phytogenic
dc.subjectApoptosis
dc.subjectBreast Neoplasms
dc.subjectCaspase 3
dc.subjectCaspase 7
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectDNA Fragmentation
dc.subjectDNA Methylation
dc.subjectEpigenesis, Genetic
dc.subjectHistone Deacetylases
dc.subjectHumans
dc.subjectMCF-7 Cells
dc.subjectPlant Extracts
dc.subjectThymus Plant
dc.subjectantineoplastic agent
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectDNA methyltransferase
dc.subjecthistone deacetylase
dc.subjectplant extract
dc.subjectthymus serpyllum extract
dc.subjectunclassified drug
dc.subjectantineoplastic activity
dc.subjectapoptosis
dc.subjectarticle
dc.subjectbreast cancer
dc.subjectcancer cell
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectDNA fragmentation
dc.subjectdrug cytotoxicity
dc.subjectdrug effect
dc.subjectdrug inhibition
dc.subjectdrug response
dc.subjectenzyme activity
dc.subjectenzyme linked immunosorbent assay
dc.subjectepigenetics
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmedicinal plant
dc.subjectreverse transcription polymerase chain reaction
dc.subjectThymus (plant)
dc.subjectthymus serpyllum
dc.titleEffects of Thymus serpyllum extract on cell proliferation, apoptosis and epigenetic events in human breast cancer Cells
dc.typeArticle

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