Significant changes in trans-epithelial barrier proteins of adenoid tissue with atopic status in children

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dc.contributor.authorYılmaz Ö.
dc.contributor.authorŞimşek Y.
dc.contributor.authorİnan S.
dc.contributor.authorBuga Ö.
dc.contributor.authorEskiizmir G.
dc.contributor.authorPınar E.
dc.contributor.authorKanık E.
dc.contributor.authorYüksel H.
dc.date.accessioned2024-07-22T08:08:00Z
dc.date.available2024-07-22T08:08:00Z
dc.date.issued2020
dc.description.abstractOBJECTIVES: Adenoid tissue is important in local immune response and epithelial barrier dysfunction of this tissue may contribute to allergies. The aim of this study was to evaluate the relationship between the status of cross-epithelial barrier elements in adenoid tissue lymphoepithelium and inhalant allergen sensitization. MATERIALS AND METHODS: Children aged 5-15 years, who underwent adenotonsillectomy, participated in this study. All subjects underwent skin prick testing with environmental inhalant allergens. Occludin, ZO1, e-cadherin, β-catenin, desmoglein, desmoplakin, and connexon-43 were stained immunohistochemically in the adenoid tissues obtained and scored by H-score. RESULTS: We enrolled 76 children, 14 among whom were sensitized to environmental allergens. Among the zonula occludens proteins, median H-scores for occludin, claudin, and ZO-1 were significantly lower in the atopic compared to the nonatopic group respectively (p<0.001). Similarly, median H-scores for e-cadherin and β catenin proteins of the zonula adherens were significantly lower in the atopic group (p<0.001). Both desmoglein and desmoplakin H-scores were significantly lower in the atopic group [60 (50-100) vs 280 (260300), p<0.001 and 105 (87.5-120) vs 280 (67.25-300), p<0.001 respectively]. Moreover, connexin-43 protein of the gap junction was significantly lower in the atopic group (p<0.001). CONCLUSION: Adenoid tissue, which is the initial point of contact of inhalant allergens demonstrates epithelial barrier junctional protein, changes in children with inhalant allergen sensitization without clinical allergic disease symptoms. Therefore, it may be concluded that epithelial barrier function plays an important role in the development of allergen sensitization versus tolerance. © 2020 by Turkish Thoracic Society.
dc.identifier.DOI-ID10.5152/TurkThoracJ.2019.18207
dc.identifier.issn21492530
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/14205
dc.language.isoEnglish
dc.publisherAVES
dc.rightsAll Open Access; Bronze Open Access; Green Open Access
dc.subjectallergen
dc.subjectbeta catenin
dc.subjectcadherin
dc.subjectcell adhesion molecule
dc.subjectcell membrane protein
dc.subjectconnexin 43
dc.subjectdesmoglein
dc.subjectdesmoplakin
dc.subjectoccludin
dc.subjecttrans epithelial barrier protein
dc.subjectunclassified drug
dc.subjectadenotonsillectomy
dc.subjectadherens junction
dc.subjectadolescent
dc.subjectallergic disease
dc.subjectArticle
dc.subjectatopy
dc.subjectbreathing disorder
dc.subjectchild
dc.subjectcross-sectional study
dc.subjectcytoskeleton
dc.subjectdendritic cell
dc.subjectDermatophagoides
dc.subjectdesmosome
dc.subjectforced vital capacity
dc.subjectgrass pollen
dc.subjecthistopathology
dc.subjecthomeostasis
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmune response
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectinnate immunity
dc.subjectmajor clinical study
dc.subjectolive tree
dc.subjectotitis media
dc.subjectpreschool child
dc.subjectprick test
dc.subjectschool child
dc.subjecttight junction
dc.titleSignificant changes in trans-epithelial barrier proteins of adenoid tissue with atopic status in children
dc.typeArticle

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