Effect of transtympanic betamethasone delivery to the inner ear

Simple item page
dc.contributor.authorÖzel H.E.
dc.contributor.authorÖzdoğan F.
dc.contributor.authorGülşen Gürgen S.
dc.contributor.authorEsen E.
dc.contributor.authorSelçuk A.
dc.contributor.authorGenç S.
dc.date.accessioned2024-07-22T08:11:37Z
dc.date.available2024-07-22T08:11:37Z
dc.date.issued2016
dc.description.abstractTo investigate the effect of transtympanic betamethasone administration on hearing function with histologic correlation, rats were divided into three transtympanic treatment groups: isotonic saline (group I, n = 10), gentamicin (group II, n = 10) and betamethasone (group III, n = 10). Distortion product otoacoustic emission thresholds were compared on day 10. Also histological effects on cellular apoptosis in both the inner and outer hair cells in organ of Corti and spiral ganglion neurons were evaluated. Distortion product otoacoustic emission thresholds were comparable (p > 0.05) between group I and group III in all measurements. Distortion product otoacoustic emission thresholds of group II were significantly elevated in all measurements when compared with group I (p < 0.05) and group III (p < 0.05). In the Terminal deoxynucleotidyl transferase dUTP Nick End Labelling (TUNEL), Caspase-3, Caspase-8 and Caspase-9 staining method the amount of apoptotic cells in group II were significantly elevated in all measurements compared with group I (p < 0.05). In the TUNEL staining method the amount of apoptotic cells in Group III were significantly elevated compared with group I in both the organ of Corti and spiral ganglion neurons (p < 0.05). The overall histological results revealed that the severity of cellular apoptosis caused by betamethasone was somewhere between isotonic saline and gentamicin. Transtympanic betamethasone does not affect inner ear function as measured by distortion product otoacoustic emission responses, but some increase in cellular apoptosis in the organ of Corti and spiral ganglion neurons was observed. These findings suggest that transtympanic betamethasone may have mild ototoxic effects. Further studies are needed to obtain precise results for transtympanic application of betamethasone. © 2016, Springer-Verlag Berlin Heidelberg.
dc.identifier.DOI-ID10.1007/s00405-016-3905-9
dc.identifier.issn09374477
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15722
dc.language.isoEnglish
dc.publisherSpringer Verlag
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectBetamethasone
dc.subjectCaspase 3
dc.subjectCaspase 8
dc.subjectCochlea
dc.subjectEar, Inner
dc.subjectGentamicins
dc.subjectGlucocorticoids
dc.subjectHair Cells, Auditory, Outer
dc.subjectIn Situ Nick-End Labeling
dc.subjectMale
dc.subjectOrgan of Corti
dc.subjectOtoacoustic Emissions, Spontaneous
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectSensory Thresholds
dc.subjectSpiral Ganglion
dc.subjectTympanic Membrane
dc.subjectbetamethasone
dc.subjectcaspase 3
dc.subjectcaspase 8
dc.subjectcaspase 9
dc.subjectgentamicin
dc.subjectsodium chloride
dc.subjectbetamethasone
dc.subjectcaspase 3
dc.subjectcaspase 8
dc.subjectgentamicin
dc.subjectglucocorticoid
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectCorti organ
dc.subjectdistortion product otoacoustic emission
dc.subjectdrug delivery system
dc.subjectdrug effect
dc.subjecthistology
dc.subjecthistopathology
dc.subjectimmunohistochemistry
dc.subjectinner ear
dc.subjectmale
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectrat
dc.subjectspiral ganglion
dc.subjectTUNEL assay
dc.subjectanimal
dc.subjectcochlea
dc.subjectdrug effects
dc.subjecteardrum
dc.subjectenzymology
dc.subjectinner ear
dc.subjectmetabolism
dc.subjectouter hair cell
dc.subjectperceptive threshold
dc.subjectphysiology
dc.subjectspontaneous otoacoustic emission
dc.subjectWistar rat
dc.titleEffect of transtympanic betamethasone delivery to the inner ear
dc.typeArticle

Files

Collections

Scopus Koleksiyonu