Characterization of rpoB mutations by line probe assay in rifampicin-resistant Mycobacterium tuberculosis clinical isolates from the Aegean region in Turkey

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dc.contributor.authorOzkutuk N.
dc.contributor.authorGazi H.
dc.contributor.authorSurucuoglu S.
dc.contributor.authorGunduz A.
dc.contributor.authorOzbakkaloglu B.
dc.date.accessioned2024-07-22T08:22:43Z
dc.date.available2024-07-22T08:22:43Z
dc.date.issued2007
dc.description.abstractThe nature and frequency of mutations in the rpoB gene of rifampicin (RIF)-resistant Mycobacterium tuberculosis clinical isolates vary considerably according to the geographical location, and very little information is available regarding specific mutational patterns in our country. The main objective of this study was to determine the frequency of mutations in the hypervariable region of the rpoB gene in RIF-resistant M. tuberculosis isolates recovered from tuberculosis patients in our region by using the INNO-LiPA Rif. TB kit and to evaluate the performance of the kit for the detection of RIF-resistance. Mutations associated with RIF resistance were studied by line probe assay (LiPA) in 65 RIF-resistant and 56 RIF-susceptible M. tuberculosis strains isolated from different patients in the Aegean region of Turkey. The LiPA identified all susceptible strains (100%) as RIF-sensitive and 63 of 65 (96.9%) phenotypically documented RIF-resistant M. tuberculosis isolates as RIF-resistant, with specific detection of mutation in 44 (67.7%) isolates, whilst 2 strains were identified as RIF-susceptible. The R5-pattern (Ser-531-Leu mutation) was the most frequently observed (35 of 65, 53.8%), followed by the ΔS2-pattern (7.7%) and ΔS4-pattern (7.7%).
dc.identifier.issn13446304
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19196
dc.language.isoEnglish
dc.subjectAntibiotics, Antitubercular
dc.subjectBacterial Proteins
dc.subjectDrug Resistance, Bacterial
dc.subjectHumans
dc.subjectMolecular Diagnostic Techniques
dc.subjectMutation
dc.subjectMycobacterium tuberculosis
dc.subjectRifampin
dc.subjectTuberculosis
dc.subjectTurkey
dc.subjectaspartic acid
dc.subjecthistidine
dc.subjectleucine
dc.subjectrifampicin
dc.subjectRNA polymerase beta subunit
dc.subjectserine
dc.subjecttyrosine
dc.subjectvaline
dc.subjectamino acid substitution
dc.subjectantibiotic resistance
dc.subjectantibiotic sensitivity
dc.subjectarticle
dc.subjectbacterial strain
dc.subjectbacterium isolate
dc.subjectbacterium isolation
dc.subjectcontrolled study
dc.subjectgene frequency
dc.subjectgene identification
dc.subjectgene mutation
dc.subjectgeographic distribution
dc.subjecthuman
dc.subjectimmunoglobulin variable region
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectnucleic acid base substitution
dc.subjecttuberculosis
dc.subjectTurkey (republic)
dc.titleCharacterization of rpoB mutations by line probe assay in rifampicin-resistant Mycobacterium tuberculosis clinical isolates from the Aegean region in Turkey
dc.typeArticle

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