Histologic and morphologic effects of valproic acid and oxcarbazepine on rat uterine and ovarian cells

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dc.contributor.authorCansu A.
dc.contributor.authorErdogan D.
dc.contributor.authorSerdaroglu A.
dc.contributor.authorTake G.
dc.contributor.authorCoskun Z.K.
dc.contributor.authorGurgen S.G.
dc.date.accessioned2024-07-22T08:21:01Z
dc.date.available2024-07-22T08:21:01Z
dc.date.issued2010
dc.description.abstractPurpose: To determine the histologic and morphologic effects of valproic acid (VPA) and oxcarbazepine (OXC) on rat uterine and ovarian cells. Methods: Fifty-six female prepubertal Wistar rats (21-24 days old and weighing between 47.5 and 58.1 g) were divided equally into four groups, which were given drinking water (controls), 300 mg/kg/day of VPA, 100 mg/kg/day of OXC or VPA + OXC via gavage, for 90 days. Ovaries and uteri of rats on proestrous and diestrous phases of estrous cycle were extirpated and placed in a fixation solution. The tissue specimens were assessed with apoptosis (TUNEL) staining protocols, eosinophil counting, and electron microscopic techniques. Results: In uteri, apoptosis in stroma, mitochondrial swelling, and cristolysis were observed in the VPA group, and OXC led to negative effects on epithelial cell and intracellular edema. In ovaries, both drugs increased apoptosis and intracytoplasmic edema. Organelle structure disruption was also observed in the OXC group. More conspicuous degenerative modifications were determined in the VPA + OXC group. In uteri, the number of TUNEL-positive luminal epithelial cells was 7.20 ± 1.32 in controls, and significantly increased to 29.60 ± 1.58, 34.20 ± 2.53, and 54.80 ± 2.04 in VPA, OXC, and VPA + OXC groups, respectively (p < 0.001). The highest number of TUNEL-positive glandular epithelium cells was observed in the VPA + OXC group; however, the number of TUNEL-positive stroma cells was highest in the VPA group. The highest number of eosinophils in stroma was in the VPA group. Conclusion: VPA and OXC trigger apoptotic and degenerative effects on rat uterine and ovarian cells. VPA also prevents implantation of embryo to the uterus and causes abortion via endometrial eosinophil infiltration. © 2009 International League Against Epilepsy.
dc.identifier.DOI-ID10.1111/j.1528-1167.2009.02259.x
dc.identifier.issn15281167
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18426
dc.language.isoEnglish
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAnimals
dc.subjectAnticonvulsants
dc.subjectApoptosis
dc.subjectCarbamazepine
dc.subjectCell Count
dc.subjectCytoplasm
dc.subjectEmbryo Implantation
dc.subjectEndometrium
dc.subjectEosinophils
dc.subjectEpithelial Cells
dc.subjectEpithelium
dc.subjectEstrous Cycle
dc.subjectFemale
dc.subjectIn Situ Nick-End Labeling
dc.subjectMicroscopy, Electron
dc.subjectMitochondrial Swelling
dc.subjectOvary
dc.subjectPregnancy
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectUterus
dc.subjectValproic Acid
dc.subjectoxcarbazepine
dc.subjectvalproic acid
dc.subjectabortion
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectembryo
dc.subjecteosinophil count
dc.subjectepithelium cell
dc.subjectfemale
dc.subjecthistology
dc.subjectmitochondrion swelling
dc.subjectmorphology
dc.subjectnonhuman
dc.subjectovary cell
dc.subjectpriority journal
dc.subjectrat
dc.subjectreproductive toxicity
dc.subjectstroma cell
dc.subjectuterus
dc.subjectWistar rat
dc.titleHistologic and morphologic effects of valproic acid and oxcarbazepine on rat uterine and ovarian cells
dc.typeArticle

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