Comparison of Manzanilla and wild type olives by RAPD-PCR analysis

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dc.contributor.authorSesli M.
dc.contributor.authorYeǧenoǧlu E.D.
dc.date.accessioned2024-07-22T08:21:03Z
dc.date.available2024-07-22T08:21:03Z
dc.date.issued2010
dc.description.abstractThe object of this study was to detect genetic similarities and distances between cultivated Manzanilla and wild type olives by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique. In this study, the cultivated type olive Manzanilla was supplied from Olive Research Institute of Turkey and the wild type olives from the villages of Manisa, Izmir and Mugla provinces. Genomic DNA's were extracted from young leaves and PCR was used to generate RAPD bands. Sixty random primers obtained from Operon technology were tested by RAPD-PCR (OP-A, OP-I, OP-Q). Forty one of 60 primers used in the study provided 346 considerably polymorphic bands and the average number of bands was defined as 8.43. Comparisons of cultivated and wild type olives are important for understanding genetic relation. It was determined that the genetic distance values varied between 0.0665 and 0.2863 and genetic similarity values varied between 0.9356 and 0.7511. It was observed that the samples most close to each other were wild 8 and wild 3 and the samples most distant from each other were wild 5 and Manzanilla, in accordance with such genetic distance and similarity values. © 2010 Academic Journals.
dc.identifier.DOI-ID10.5897/ajb09.1682
dc.identifier.issn16845315
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18445
dc.language.isoEnglish
dc.publisherAcademic Journals
dc.rightsAll Open Access; Green Open Access; Hybrid Gold Open Access
dc.subjectOlea europaea var. sylvestris
dc.subjectOleaceae
dc.subjectgenomic DNA
dc.subjectagar gel electrophoresis
dc.subjectarticle
dc.subjectcomparative study
dc.subjectDNA extraction
dc.subjectgenetic distance
dc.subjectgenetic similarity
dc.subjectmanzanilla
dc.subjectmolecular phylogeny
dc.subjectnonhuman
dc.subjectolive
dc.subjectplant
dc.subjectplant leaf
dc.subjectpolymerase chain reaction
dc.subjectrandom amplified polymorphic DNA
dc.subjectspectrophotometry
dc.subjecttillage
dc.subjectwild type
dc.titleComparison of Manzanilla and wild type olives by RAPD-PCR analysis
dc.typeArticle

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