Combined gossypol and zoledronic acid treatment results in synergistic induction of cell death and regulates angiogenic molecules in ovarian cancer cells

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dc.contributor.authorAtmaca H.
dc.contributor.authorGorumlu G.
dc.contributor.authorKaraca B.
dc.contributor.authorDegirmenci M.
dc.contributor.authorTunali D.
dc.contributor.authorCirak Y.
dc.contributor.authorPurcu D.U.
dc.contributor.authorUzunoglu S.
dc.contributor.authorKarabulut B.
dc.contributor.authorSanli U.A.
dc.contributor.authorUslu R.
dc.date.accessioned2024-07-22T08:21:14Z
dc.date.available2024-07-22T08:21:14Z
dc.date.issued2009
dc.description.abstractIn the present study, we aimed to evaluate the possible synergistic, cytotoxic effects of combination treatment of gossypol and zoledronic acid, in human ovarian cancer cell lines, OVCAR-3 and MDAH-2774, and to elucidate the role of this novel combination treatment on angiogenesis-related molecules in ovarian cancer. The XTT cell viability assay was used for showing cytotoxicity. Both DNA fragmentation by ELISA assay and caspase 3/7 activity measurement were used for demonstrating apoptosis. To elucidate the angiogenic molecules affected by combination treatment, mRNA levels of angiogenic molecules were measured using the Human Angiogenesis RT2 Profiler™ PCR Array (SuperArray, Frederick, MD) in ovarian cancer cell lines, OVCAR-3 and MDAH-2774.The combined treatment resulted in significant, synergistic cytotoxicity, and induced apoptosis. This effect was observed to happen in a dose- and time-dependent manner. Moreover, the combination treatment of 10 μM gossypol and 5 μM zoledronic acid resulted in significant down-regulation (≥ thee-fold) in mRNA levels of some pivotal angiogenic molecules in OVCAR-3 and MDAH-2774 cells as compared to the untreated control. However, this effect was different in the two ovarian cancer cell lines observed. Gossypol, in combination with zoledronic acid, may provide a rational treatment option for ovarian cancer, not only by direct inhibition of cell proliferation, but also inhibition of angiogenesis-related molecules.
dc.identifier.DOI-ID10.1684/ecn.2009.0159
dc.identifier.issn11485493
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/18522
dc.language.isoEnglish
dc.subjectAngiogenesis Inducing Agents
dc.subjectCaspase 3
dc.subjectCaspase 7
dc.subjectCell Death
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectDiphosphonates
dc.subjectDNA Fragmentation
dc.subjectDown-Regulation
dc.subjectDrug Resistance, Neoplasm
dc.subjectDrug Screening Assays, Antitumor
dc.subjectDrug Synergism
dc.subjectFemale
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectGossypol
dc.subjectHumans
dc.subjectImidazoles
dc.subjectOvarian Neoplasms
dc.subjectRNA, Messenger
dc.subjectcaspase 3
dc.subjectcaspase 7
dc.subjectgossypol
dc.subjectmessenger RNA
dc.subjectzoledronic acid
dc.subjectangiogenesis
dc.subjectapoptosis
dc.subjectarticle
dc.subjectcancer cell culture
dc.subjectcell death
dc.subjectcell proliferation
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectDNA fragmentation
dc.subjectdown regulation
dc.subjectdrug potentiation
dc.subjectenzyme activity
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjecthuman cell
dc.subjectIC 50
dc.subjectovary cancer
dc.titleCombined gossypol and zoledronic acid treatment results in synergistic induction of cell death and regulates angiogenic molecules in ovarian cancer cells
dc.typeArticle

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