Up-regulation of serine/threonine protein phosphatase type 2A regulatory subunits during methylprednisolone-induced differentiation of leukaemic HL-60 cells
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Date
2000
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Abstract
Serine/threonine protein phosphatase 2A (PP2A) may play a role in leukaemic cell differentiation of the HL 60 myeloid leukaemic cell-line after methylprednisolone induction. We have investigated the specific enzyme activity and expression of catalytic and regulatory subunits of PP2A. The resulting specific enzyme activity and immunoblots showed an increase in enzyme activity and the expression of regulatory subunits after methylprednisolone treatment. There was no change in the expression of PP2A catalytic subunits. It is suggested that the effect of methylprednisolone on leukaemic differentiation may be the result of PP2 A upregulation.
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Anti-Inflammatory Agents , Blotting, Western , Cell Differentiation , Cytosol , HL-60 Cells , Humans , Methylprednisolone , Phosphoprotein Phosphatase , Protein Subunits , Time Factors , Up-Regulation , methylprednisolone , phosphatase , serine derivative , serine threonine protein phosphatase 2a , unclassified drug , article , catalysis , cell differentiation , cell line , controlled study , enzyme activity , human , human cell , immunoblotting , leukemia cell , myeloid leukemia , priority journal , protein expression , receptor upregulation