Long term neuroprotective effects of acute single dose MK-801treatment against traumatic brain injury in immature rats

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dc.contributor.authorCigel A.
dc.contributor.authorSayin O.
dc.contributor.authorGurgen S.G.
dc.contributor.authorSonmez A.
dc.date.accessioned2024-07-22T08:05:37Z
dc.date.available2024-07-22T08:05:37Z
dc.date.issued2021
dc.description.abstractBecause brain development continues during adolescence, childhood trauma is a major health problem in pediatric ages. It is known traumatic brain injury (TBI) results in damage in hippocampal and cortical areas of the brain and impairs cognitive functions. The study aims to investigate the long-term effects of MK-801 (dizocilpine), an N-methyl D-aspartate (NMDA) receptor antagonist, on hippocampal damage, locomotor activity, and cognitive functions following TBI in immature rats. MK-801 (1 mg/kg) was injected intraperitoneally immediately after TBI. Thirty-seven litters were randomly allocated into three groups at 7 days (P7) of postnatal age: a control group, a trauma group, and an MK-801 treatment group. The control group received no treatment; the trauma group received saline as vehicle control for the MK-801 group and the MK-801 group received a single dose of 1 mg/kg MK-801 immediately after TBI. Hippocampal damage was examined by Hematoxylin-Eosin staining. Brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), NMDA-R, and glial fibrillar acidic protein (GFAP) immunohistochemistry and, BDNF, NGF, and NMDA-R ELISA protein levels were evaluated 125 days after trauma. Histopathological and immunohistochemical evaluations showed that treatment with MK-801 significantly ameliorated the trauma-induced hippocampal neuron loss and increased BDNF, NGF, NMDA-R, GFAP expressions in CA1, CA3, and DG hippocampal regions. Additionally, treatment with MK-801 decreased anxiety and increased hippocampus-dependent memory of animals subjected to brain injury after TBI. These results show that acute treatment of MK-801 has a neuroprotective role against trauma-induced hippocampal neuron loss and associated cognitive impairment in rats. © 2021 Elsevier Ltd
dc.identifier.DOI-ID10.1016/j.npep.2021.102161
dc.identifier.issn01434179
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13199
dc.language.isoEnglish
dc.publisherChurchill Livingstone
dc.subjectAnimals
dc.subjectBrain
dc.subjectBrain Injuries, Traumatic
dc.subjectDizocilpine Maleate
dc.subjectExcitatory Amino Acid Antagonists
dc.subjectHippocampus
dc.subjectNeuroprotective Agents
dc.subjectRats
dc.subjectTime
dc.subjectbrain derived neurotrophic factor
dc.subjectdizocilpine
dc.subjectglial fibrillary acidic protein
dc.subjectn methyl dextro aspartic acid receptor
dc.subjectnerve growth factor
dc.subjectamino acid receptor blocking agent
dc.subjectdizocilpine maleate
dc.subjectneuroprotective agent
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectclinical effectiveness
dc.subjectcontrolled study
dc.subjectdose response
dc.subjectdrug effect
dc.subjectdrug efficacy
dc.subjectdrug mechanism
dc.subjectenzyme linked immunosorbent assay
dc.subjecthippocampal CA1 region
dc.subjecthippocampal CA3 region
dc.subjectimmunohistochemistry
dc.subjectneuroprotection
dc.subjectnonhuman
dc.subjectperinatal period
dc.subjectprotein expression
dc.subjectrat
dc.subjectsingle drug dose
dc.subjectspinal ganglion
dc.subjectSprague Dawley rat
dc.subjecttraumatic brain injury
dc.subjecttreatment outcome
dc.subjectanimal
dc.subjectbrain
dc.subjecthippocampus
dc.subjectmetabolism
dc.subjecttime
dc.subjecttraumatic brain injury
dc.titleLong term neuroprotective effects of acute single dose MK-801treatment against traumatic brain injury in immature rats
dc.typeArticle

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