Does Pten have an impact on oogenesis of PCOS mouse models?

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dc.contributor.authorOnal T.
dc.contributor.authorTulay P.
dc.contributor.authorVatansever H.S.
dc.date.accessioned2024-07-22T08:03:06Z
dc.date.available2024-07-22T08:03:06Z
dc.date.issued2023
dc.description.abstractPolycystic ovary syndrome (PCOS) is a complex disorder in which the aetiology is still not explained very well. The PI3K/PTEN (phosphatidylinositol 3-kinase/phosphatase and tensin homolog deleted on chromosome 10) pathway is an important pathway that is involved in many mechanisms, including proliferation, growth and motility. PTEN plays a role in granulosa cell proliferation and regulates the differentiation process. The aim of this study was to investigate the expression levels of Pten and Pik3ca in PCOS mouse models with and without any treatment procedures. Three groups of mouse models, PCOS, a PCOS group with clomiphene citrate treatment, and a PCOS group with the combination of clomiphene citrate, metformin and pioglitazone treatment, were established. Ovarian tissues, which were obtained from these groups and a control group with no PCOS, were embedded in paraffin and RNA was extracted. cDNA was synthesized and real-time PCR was conducted to evaluate the expression levels of Pten and Pik3ca. The results of this study showed that both Pten and Pik3ca genes were expressed in the ovarian tissues from the mouse models. Although one-way analysis of variance results showed that Pten was expressed significantly differently in the samples, individual Student's t-tests did not show any significantly different expression levels in each group. This study is important as it shows the expression patterns of two genes in PCOS mouse models with different treatment strategies, including clomiphene citrate, metformin and pioglitazone. The results of this study formed the basis of research studies and investigations into different genes within the PTEN pathway, as well as other pathways that are under investigation. © The Author(s), 2022. Published by Cambridge University Press.
dc.identifier.DOI-ID10.1017/S0967199422000661
dc.identifier.issn09671994
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12129
dc.language.isoEnglish
dc.publisherCambridge University Press
dc.subjectAnimals
dc.subjectClomiphene
dc.subjectFemale
dc.subjectHumans
dc.subjectMetformin
dc.subjectMice
dc.subjectOogenesis
dc.subjectOvulation Induction
dc.subjectPhosphatidylinositol 3-Kinases
dc.subjectPioglitazone
dc.subjectPolycystic Ovary Syndrome
dc.subjectPTEN Phosphohydrolase
dc.subjectclomifene
dc.subjectmetformin
dc.subjectphosphatidylinositol 3 kinase
dc.subjectphosphatidylinositol 3,4,5 trisphosphate 3 phosphatase
dc.subjectpioglitazone
dc.subjectPTEN protein, human
dc.subjectanimal
dc.subjectfemale
dc.subjectgenetics
dc.subjecthuman
dc.subjectmetabolism
dc.subjectmouse
dc.subjectoocyte development
dc.subjectovary polycystic disease
dc.subjectovulation induction
dc.subjectprocedures
dc.titleDoes Pten have an impact on oogenesis of PCOS mouse models?
dc.typeArticle

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