Therapeutic and protective effects of autologous serum in amikacin-induced ototoxicity
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Date
2018
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Abstract
Objective: Possible therapeutic and protective benefits of intratympanic autologous serum application in amikacin-induced ototoxicity were investigated. Methods: Twenty-four Guinea pigs were separated equally into two groups: therapeutic (group A) and protective (group B). Transient evoked otoacoustic emissions were recorded before and after autologous serum application. Apoptotic cells were identified in the organ of Corti, spiral limbus and spiral ganglion by the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling ('TUNEL') method. Results: Transient evoked otoacoustic emission responses at 1, 1.4 and 2.8 kHz improved without significance after autologous serum application in group A (p > 0.05). A significantly protective effect of autologous serum was determined at 4 kHz in group B (p < 0.05). There were significantly fewer apoptotic cells at the spiral limbus in the therapeutic and protective groups compared to the control group (p < 0.05). Conclusion: Autologous serum may offer protection against ototoxicity-induced hearing loss, but it cannot restore hearing. Immunohistochemically, autologous serum significantly decreases activation of the intrinsic pathway of pro-apoptotic signalling in mesenchymal cells compared to neurons and neurosensory cells. © 2017 JLO (1984) Limited.
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Keywords
Amikacin , Animals , Apoptosis , Blood Component Transfusion , Disease Models, Animal , Female , Guinea Pigs , Hearing Disorders , Immunohistochemistry , Otoacoustic Emissions, Spontaneous , Serum , Spiral Ganglion , Treatment Outcome , amikacin , Fas ligand , protein Bax , amikacin , animal experiment , apoptosis , Article , autologous serum , controlled study , Corti organ , evoked otoacoustic emission , female , guinea pig , histopathology , immunohistochemistry , immunoreactivity , nonhuman , otoacoustic emission , ototoxicity , serum , signal noise ratio , spiral ganglion , TUNEL assay , animal , blood component therapy , chemically induced , disease model , drug effects , hearing disorder , pathology , pathophysiology , procedures , spontaneous otoacoustic emission , treatment outcome