Effects of cyclooxygenase on the urothelium of the urinary bladder of mice exposed to pelvic radiation

Simple item page
dc.contributor.authorOzbilgin M.K.
dc.contributor.authorOnal T.
dc.contributor.authorOzcan C.
dc.contributor.authorTemel M.
dc.contributor.authorAktas C.
dc.contributor.authorGareveran M.S.
dc.contributor.authorUluer E.T.
dc.contributor.authorInan S.
dc.contributor.authorKurtman C.
dc.date.accessioned2024-07-22T08:11:51Z
dc.date.available2024-07-22T08:11:51Z
dc.date.issued2016
dc.description.abstractOBJECTIVE: To determine the role of cyclooxygenase (COX) expression in the urothelium of the urinary bladder during radiation injury caused by pelvic radiotherapy for cancer therapy. STUDY DESIGN: Twenty-four male Swiss Albino mice were separated into 4 groups. The first group was the control group (Group 1) and the second, third, and fourth groups were euthanized after 24 hours (Group 2), 48 hours (Group 3), and 7 days (Group 4), respectively. A single-fractioned 10 Gy of ionizing radiation was applied to all mice's pelvic zone with Co-60. Bladders were removed completely from the pelvic region. Histochemical analysis using hematoxylin and eosin and immunohistochemical analysis using anti-COX-1 and COX-2 antibodies were performed on tissue samples. The immunoreactivities of the urinary bladder were quantified using H-score measurement, and statistical comparison was performed. RESULTS: In the immunohistochemical examination the COX-1 immunoreactivities were found to be higher in the urothelium of the bladder in the radiation ex-posed groups than in the normal control group (group 1) (p<0.005). Additionally, high immunoreactivity of COX-2 molecule was established in groups 2, 3, and 4 of radiation groups as compared to group 1 (p<0.005) in examination of the urothelium. COX-1 and COX-2 immunoreactivities in the submucosa were detected higher in group 4 than in the other groups (p<0.005). CONCLUSION: COX-1 and COX-2 expressions in the urothelium and subepithelium of the urinary bladder were investigated in mice during the acute radiation response. The expression of COX-1 and COX-2 in the urothelium seems to prevent bladder damage from radi-ation, supplying differentiation and restoration of the urothelium. © Science Printers and Publishers, Inc.
dc.identifier.issn08846812
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15801
dc.language.isoEnglish
dc.publisherScience Printers and Publishers Inc.
dc.subjectAnimals
dc.subjectCyclooxygenase 1
dc.subjectCyclooxygenase 2
dc.subjectMale
dc.subjectMembrane Proteins
dc.subjectMice
dc.subjectUrinary Bladder
dc.subjectUrothelium
dc.subjectcyclooxygenase 1
dc.subjectcyclooxygenase 2
dc.subjecteosin
dc.subjecthematoxylin
dc.subjectcyclooxygenase 1
dc.subjectcyclooxygenase 2
dc.subjectmembrane protein
dc.subjectPtgs1 protein, mouse
dc.subjectPtgs2 protein, mouse
dc.subjectadult
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbladder
dc.subjectbladder injury
dc.subjectbladder tissue
dc.subjectcancer radiotherapy
dc.subjectcell differentiation
dc.subjectcontrolled study
dc.subjectgene expression
dc.subjecthistochemistry
dc.subjectimmunohistochemistry
dc.subjectimmunoreactivity
dc.subjectionizing radiation
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpathophysiology
dc.subjectpelvis cancer
dc.subjectpriority journal
dc.subjectradiation exposure
dc.subjectradiation injury
dc.subjecturothelium
dc.subjectbladder
dc.subjectbladder examination
dc.subjectcancer radiotherapy
dc.subjectH score
dc.subjecthistopathology
dc.subjectpelvis radiography
dc.subjectprotein expression
dc.subjectradiation dose
dc.subjectradiation exposure
dc.subjectscoring system
dc.subjectsubmucosa
dc.subjectSwiss Webster mouse
dc.subjecturothelium
dc.subjectanimal
dc.subjectenzymology
dc.subjectmetabolism
dc.subjectpathology
dc.subjectradiation response
dc.titleEffects of cyclooxygenase on the urothelium of the urinary bladder of mice exposed to pelvic radiation
dc.typeArticle

Files

Collections

Scopus Koleksiyonu