Partial purification and characterization of polyphenoloxidase from peppermint (Mentha piperita)

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dc.contributor.authorKavrayan D.
dc.contributor.authorAydemir T.
dc.date.accessioned2024-07-22T08:25:24Z
dc.date.available2024-07-22T08:25:24Z
dc.date.issued2001
dc.description.abstractPolyphenoloxidase (PPO) of peppermint leaves ( Mentha piperita) was isolated by (NH4)2SO4 precipitation and dialysis. Its pH and temperature optima were 7.0 and 30°C, respectively. On heat-inactivation, half of the activity was lost after 6.5 and 1.5 min of treatment at 70 and 80°C, respectively. Sucrose, (NH4)2SO4, NaCl and KCl appeared to be protective agents of peppermint PPO against thermal denaturation. Km of this enzyme ranged from 6.25×10-3 M with catechol to 9.00×10-3 M with L-dopa. The I50 values of inhibitors studied on PPO were determined by means of activity percentage (I) diagrams. Values were 1.4×10-4 M, 1.7×10-4 M, 9.7×10-5 M, 2.45×10-4 M, 2.16×10-1 M, 1.83×10-5 M, 6.5×10-5 M, 1.4×10-2 M, 7.5×10-5 M, for potassium cyanide, glutathione, ascorbic acid, thiourea, sodium azide, sodium metabisulfite, dithioerythritol, β-mercaptoethanol and sodium diethyl dithiocarbamate respectively. Therefore, sodium metabisulfite was the most effective inhibitor. Copyright © 2001 Elsevier Science Ltd.
dc.identifier.DOI-ID10.1016/S0308-8146(01)00106-6
dc.identifier.issn03088146
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/20398
dc.language.isoEnglish
dc.subjectMentha x piperita
dc.subjectascorbic acid
dc.subjectcatechol oxidase
dc.subjectdiethyldithiocarbamic acid
dc.subjectdithioerythritol
dc.subjectglutathione
dc.subjectmercaptoethanol
dc.subjectpeppermint
dc.subjectpotassium cyanide
dc.subjectsodium azide
dc.subjectsodium metabisulfite
dc.subjectstabilizing agent
dc.subjectthiourea
dc.subjectarticle
dc.subjectenzyme activity
dc.subjectenzyme analysis
dc.subjectenzyme purification
dc.subjectheating
dc.subjectin vitro study
dc.subjectphytochemistry
dc.subjectplant
dc.subjectstorage
dc.titlePartial purification and characterization of polyphenoloxidase from peppermint (Mentha piperita)
dc.typeArticle

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