Leishmaniasis in Turkey: First clinical isolation of Leishmania major from 18 autochthonous cases of cutaneous leishmaniasis in four geographical regions

Simple item page
dc.contributor.authorÖzbilgin A.
dc.contributor.authorÇulha G.
dc.contributor.authorUzun S.
dc.contributor.authorHarman M.
dc.contributor.authorTopal S.G.
dc.contributor.authorOkudan F.
dc.contributor.authorZeyrek F.
dc.contributor.authorGündüz C.
dc.contributor.authorÖstan I.
dc.contributor.authorKarakuş M.
dc.contributor.authorTöz S.
dc.contributor.authorKurt Ö.
dc.contributor.authorAkyar I.
dc.contributor.authorErat A.
dc.contributor.authorGüngör D.
dc.contributor.authorKayabaşi Ç.
dc.contributor.authorÇavuş I.
dc.contributor.authorBastien P.
dc.contributor.authorPratlong F.
dc.contributor.authorKocagöz T.
dc.contributor.authorÖzbel Y.
dc.date.accessioned2024-07-22T08:11:50Z
dc.date.available2024-07-22T08:11:50Z
dc.date.issued2016
dc.description.abstractObjective: To report isolation of Leishmania major strains obtained from 18 Turkish autochthonous cutaneous leishmaniasis (CL) patients infected with L. major between 2011 and 2014. Methods: Initial diagnosis relied on microscopy and culture in enriched medium, prepared by adding specific amounts of liver extract, protein and lipid sources to NNN medium. Promastigotes were then transferred to RPMI medium including 10% of foetal calf serum for mass culture. Species-specific real-time PCR targeting ITS1 region of Leishmania spp. was performed using both lesion aspiration samples and cultured promastigotes. Two of 18 isolates were identified by isoenzyme analysis in the Leishmaniasis Reference Center in Montpellier, France. Each isolate was inoculated into the footpads of six mice to observe the pathogenicity of L. major. Developing lesions were observed, and the thickening of footpads was measured weekly. Results: Melting curve analyses of 18 isolates showed a peak concordant with L. major, and two of them were confirmed by isoenzyme analyses as L. major zymodeme MON103. In the mouse model, acute lesions seen on day 21 were accepted as an indication of heavy infection. Severe impairments were observed on all mouse footpads over 3 weeks, which even progressed to extremity amputation. Conclusion: Cutaneous leishmaniasis-causing L. major was recently identified in Adana province in southern Turkey, with PCR. Our study shows that such CL cases are not limited to Adana but currently present from western to Southeastern Anatolia, and along the Mediterranean coast. The role of small mammals, the main reservoirs of L. major in Anatolia, needs to be elucidated, as do the underlying factors that cause severe clinical manifestations in L. major infections in Turkey, contrary to the infections in neighbouring countries. © 2016 John Wiley & Sons Ltd.
dc.identifier.DOI-ID10.1111/tmi.12698
dc.identifier.issn13602276
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15778
dc.language.isoEnglish
dc.publisherBlackwell Publishing Ltd
dc.rightsAll Open Access; Bronze Open Access
dc.subjectAnimals
dc.subjectCattle
dc.subjectDisease Vectors
dc.subjectFemale
dc.subjectIsoenzymes
dc.subjectLeishmania major
dc.subjectLeishmaniasis, Cutaneous
dc.subjectMale
dc.subjectMammals
dc.subjectMice, Inbred BALB C
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectSeverity of Illness Index
dc.subjectSkin
dc.subjectTurkey
dc.subjectAdana [Turkey]
dc.subjectAnatolia
dc.subjectFrance
dc.subjectHerault
dc.subjectLanguedoc-Roussillon
dc.subjectMontpellier
dc.subjectTurkey
dc.subjectLeishmania major
dc.subjectMammalia
dc.subjectMus
dc.subjectisoenzyme
dc.subjectcuticle
dc.subjectdisease vector
dc.subjectenzyme activity
dc.subjectleishmaniasis
dc.subjectlesion
dc.subjectlipid
dc.subjectpathogenicity
dc.subjectpolymerase chain reaction
dc.subjectprotozoan
dc.subjectrodent
dc.subjectserum
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectArticle
dc.subjectclinical article
dc.subjectcontrolled study
dc.subjectfemale
dc.subjectfetal calf serum
dc.subjectgeographic distribution
dc.subjecthuman
dc.subjectisoenzyme analysis
dc.subjectLeishmania major
dc.subjectlimb amputation
dc.subjectlowest income group
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpathogenicity
dc.subjectpromastigote
dc.subjectreal time polymerase chain reaction
dc.subjectskin leishmaniasis
dc.subjectTurkey (republic)
dc.subjectanimal
dc.subjectBagg albino mouse
dc.subjectbovine
dc.subjectdisease carrier
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectLeishmaniasis, Cutaneous
dc.subjectmammal
dc.subjectparasitology
dc.subjectpathology
dc.subjectseverity of illness index
dc.subjectskin
dc.subjectTurkey
dc.titleLeishmaniasis in Turkey: First clinical isolation of Leishmania major from 18 autochthonous cases of cutaneous leishmaniasis in four geographical regions
dc.typeArticle

Files

Collections

Scopus Koleksiyonu