The effect of oestradiol and neta on immunohistochemical staining of iNOS and eNOS in coronary arteries of ovariectomized rats

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dc.contributor.authorKoyuncu F.M.
dc.contributor.authorOzbilgin K.
dc.contributor.authorKuscu N.K.
dc.contributor.authorInan S.
dc.contributor.authorVatansever S.
dc.contributor.authorCeylan E.
dc.date.accessioned2024-07-22T08:23:24Z
dc.date.available2024-07-22T08:23:24Z
dc.date.issued2006
dc.description.abstractAim: The postmenopausal period is associated with increased risk for coronary atherosclerosis, and the effect of hormone replacement therapy in reducing this risk is controversial. Previous studies reported that nitric oxide synthetase (NOS) level might be important for the development of atherosclerosis, but no study has shown the interaction between hormone replacement therapy and endothelial NOS and inducible NOS intensity on coronary arteries yet. Our goal was to find out the immunostaining intensity of endothelial NOS and inducible NOS in ovariectomized rats which received oestradiol and norethisterone treatment. Methods: We performed bilateral ovariectomy in 15, female, 90-day-old Wistar rats with an average weight of 250 grams. After waiting for 4 weeks for the menopausal state, they were divided into 3 groups to receive either placebo, 0.1 mg/day 17-β-oestradiol (group E2), or 0.1 mg/day 17-β-oestradiol + 0.1 mg/day norethisterone acetate (group E2-NETA) for 5 weeks. Another group included 5, normal, adult, female intact rats and served as controls. At the end of the treatment, all rats were sacrificed and coronary arteries were stained with inducible NOS and endothelial NOS polyclonal antibodies using streptavidin-biotin technique. Results: The immunostaining of inducible NOS was prominent in perivascular connective tissue of the ovariectomized group but not in the control group. The inducible NOS immunostaining immunoreactivity was not detected in either treated groups. Immunostaining intensity of endothelial NOS did not differ in any 4 groups with similar staining. Conclusion: The present findings indicate that hormone replacement therapy down-regulates iNOS expression in coronary arteries of ovariectomized rats, and reduced iNOS may likely be involved in estrogen's beneficial effects.
dc.identifier.issn02133911
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/19518
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectAtherosclerosis
dc.subjectConnective Tissue
dc.subjectCoronary Vessels
dc.subjectDown-Regulation
dc.subjectEstradiol
dc.subjectEstrogen Replacement Therapy
dc.subjectEstrogens
dc.subjectFemale
dc.subjectImmunohistochemistry
dc.subjectNitric Oxide Synthase Type II
dc.subjectNitric Oxide Synthase Type III
dc.subjectNorethindrone
dc.subjectOvariectomy
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectAnimalia
dc.subjectNeta
dc.subjectRattus norvegicus
dc.subjectbiotin
dc.subjectendothelial nitric oxide synthase
dc.subjectestradiol
dc.subjectinducible nitric oxide synthase
dc.subjectnorethisterone acetate
dc.subjectplacebo
dc.subjectpolyclonal antibody
dc.subjectstreptavidin
dc.subjectdrug derivative
dc.subjectendothelial nitric oxide synthase
dc.subjectestrogen
dc.subjectinducible nitric oxide synthase
dc.subjectnorethisterone
dc.subjectnorethisterone acetate
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectcoronary artery
dc.subjectcoronary artery atherosclerosis
dc.subjectdown regulation
dc.subjectfemale
dc.subjectgene expression regulation
dc.subjecthormone substitution
dc.subjectimmunohistochemistry
dc.subjectmenopause
dc.subjectnonhuman
dc.subjectovariectomy
dc.subjectpostmenopause
dc.subjectprotein expression
dc.subjectrat
dc.subjectrat strain
dc.subjectrisk reduction
dc.subjectanimal
dc.subjectatherosclerosis
dc.subjectconnective tissue
dc.subjectcoronary blood vessel
dc.subjectcytology
dc.subjectdrug effect
dc.subjectenzymology
dc.subjectestrogen therapy
dc.subjectgenetics
dc.subjectmetabolism
dc.subjectovariectomy
dc.subjectphysiology
dc.subjectWistar rat
dc.titleThe effect of oestradiol and neta on immunohistochemical staining of iNOS and eNOS in coronary arteries of ovariectomized rats
dc.typeArticle

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