Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications
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Date
2011
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Abstract
Poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl-benzo[1,2,5] thiadiazole) (PBDT) and poly(4,7-di(2,3)-dihydrothienol[3,4-b][1,4]dioxin-5-yl- 2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at -0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (Km and Imax) and surface morphologies. The biosensor was tested on real human blood serum samples. © 2011 Elsevier B.V. Al rights reserved.
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Keywords
Conducting polymers , Electrodeposition , Enzyme electrodes , Enzyme immobilization , Glucose , Glucose oxidase , Glucose sensors , Organic conductors , Organic pollutants , Polymer membrane electrodes , Reduction , 2 ,1 ,3-benzoselenadiazole , 5]thiadiazole , Ag/AgCl , Benzoselenadiazole , Benzothiadiazoles , Biosensing , Biosensor applications , Electrochemical deposition , Enzymatic reaction , Human bloods , Immobilization matrices , Modified electrodes , Oxygen levels , Polymeric matrices , Storage stability , Substrate concentrations , Graphite electrodes