The effects of pirfenidone on T helper cells in prevention of intraperitoneal adhesions

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dc.contributor.authorOzbilgin K.
dc.contributor.authorÜner M.A.
dc.contributor.authorOzkut M.
dc.contributor.authorHasdemir P.S.
dc.date.accessioned2024-07-22T08:10:40Z
dc.date.available2024-07-22T08:10:40Z
dc.date.issued2017
dc.description.abstractAbdominal surgery is linked with peritoneal adhesions. We investigated that the anti-fibrotic agent pirfenidone (PFD) has immune modulating activities and evaluated its effects on the function of T helper type 1 (Th1), Th2 and T regulatory (Treg) cells, which may play important roles in peritoneal adhesions. Eighteen female Wistar rats underwent right-sided parietal peritoneal and right uterine horn adhesion model. Rats were randomized into 3 groups as group 1 (control) (closure of midline abdominal incision without any agent administrations), group 2 (closure of incision after intraperitoneal administration of PFD) and group 3 (closure of incision and only oral administration of PFD for 14 days). Relaparotomy was performed 14 days after the first surgery. Effect of PFD on adhesion formation was assessed on Th1, Th2 and Treg cells counts using Anti-T-bet, Anti-GATA-3 Anti-FOXP3 antibodies respectively. Th1 counts were moderate in the control group, and didn't show a significant difference between all groups. Th2 cell counts were very high in the control group, but both intraperitoneal and oral administration of PFD resulted in a significant reduction in Th2 cell counts. Treg cell counts were low in number in the control group. In the intraperitoneal administration of PFD group, Treg cell counts were significantly lower than control group. There was no difference of the Treg cells between control groups and the oral administration of PFD group. PFD has prevention effect on intraperitoneal adhesions. This prevention effect seems to be related with the reduction in the numbers of Th2 and Treg cells. © 2017
dc.identifier.DOI-ID10.1016/j.kjms.2017.03.011
dc.identifier.issn1607551X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15339
dc.language.isoEnglish
dc.publisherElsevier (Singapore) Pte Ltd
dc.rightsAll Open Access; Gold Open Access
dc.subjectAnimals
dc.subjectDrug Evaluation, Preclinical
dc.subjectFemale
dc.subjectPeritoneal Cavity
dc.subjectPyridones
dc.subjectRats, Wistar
dc.subjectT-Lymphocytes, Helper-Inducer
dc.subjectT-Lymphocytes, Regulatory
dc.subjectTissue Adhesions
dc.subjectpirfenidone
dc.subjecttranscription factor FOXP3
dc.subjecttranscription factor GATA 3
dc.subjecttranscription factor T bet
dc.subjectpirfenidone
dc.subjectpyridone derivative
dc.subjectanimal experiment
dc.subjectArticle
dc.subjectcell count
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectfemale
dc.subjectnonhuman
dc.subjectperitoneum adhesion
dc.subjectrat
dc.subjectregulatory T lymphocyte
dc.subjectT lymphocyte
dc.subjectTh1 cell
dc.subjectTh2 cell
dc.subjectuterus horn
dc.subjectanimal
dc.subjectdrug effects
dc.subjecthelper cell
dc.subjectpathology
dc.subjectperitoneal cavity
dc.subjectphysiology
dc.subjectpreclinical study
dc.subjecttissue adhesion
dc.subjectWistar rat
dc.titleThe effects of pirfenidone on T helper cells in prevention of intraperitoneal adhesions
dc.typeArticle

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