Do prion protein gene polymorphisms induce apoptosis in non-mammals?

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dc.contributor.authorBirkan T.
dc.contributor.authorŞahin M.
dc.contributor.authorÖztel Z.
dc.contributor.authorBalcan E.
dc.date.accessioned2024-07-22T08:12:09Z
dc.date.available2024-07-22T08:12:09Z
dc.date.issued2016
dc.description.abstractGenetic variations such as single nucleotide polymorphisms (SNPs) in prion protein coding gene, Prnp, greatly affect susceptibility to prion diseases in mammals. Here, the coding region of Prnp was screened for polymorphisms in red-eared turtle, Trachemys scripta. Four polymorphisms, L203V, N205I, V225A and M237V, were common in 15 out of 30 turtles; in one sample, three SNPs, L203V, N205I and M237V, and in the remaining 14 samples, only L203V and N205I polymorphisms, were investigated. Besides, C658T, C664T, C670A and C823A SNPs were silent mutations. To elucidate the relationship between the SNPs and apoptosis, TUNEL assays and active caspase-3 immunodetection techniques in brain sections of the polymorphic samples were performed. The results revealed that TUNEL-positive cells and active caspase-3-positive cells in the turtles with four polymorphisms were significantly increased compared with those of the turtles with two polymorphisms (P<0.01 and P<0.05, respectively). In conclusion, this study provides preliminary information about the possible relationship between SNPs within the Prnp locus and apoptosis in a non-mammalian species, Trachemys scripta, in which prion disease has never been reported. © 2016, Indian Academy of Sciences.
dc.identifier.DOI-ID10.1007/s12038-015-9584-7
dc.identifier.issn02505991
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15939
dc.language.isoEnglish
dc.publisherSpringer India
dc.subjectAlleles
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectCaspase 3
dc.subjectGenotype
dc.subjectHaplotypes
dc.subjectMammals
dc.subjectPolymorphism, Single Nucleotide
dc.subjectPrion Proteins
dc.subjectSequence Analysis, DNA
dc.subjectTurtles
dc.subjectMammalia
dc.subjectTestudines
dc.subjectTrachemys scripta
dc.subjectcaspase 3
dc.subjectprion protein
dc.subjectcaspase 3
dc.subjectprion protein
dc.subjectapoptosis
dc.subjectDNA
dc.subjectgene
dc.subjectpolymorphism
dc.subjectprotein
dc.subjectturtle
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcontrolled study
dc.subjectenzyme activation
dc.subjectgenetic code
dc.subjectimmunodetection
dc.subjectnonhuman
dc.subjectPrnp gene
dc.subjectsilent mutation
dc.subjectsingle nucleotide polymorphism
dc.subjectTrachemys scripta
dc.subjectTUNEL assay
dc.subjectallele
dc.subjectanimal
dc.subjectapoptosis
dc.subjectDNA sequence
dc.subjectgenetics
dc.subjectgenotype
dc.subjecthaplotype
dc.subjectmammal
dc.subjectsingle nucleotide polymorphism
dc.subjectturtle
dc.titleDo prion protein gene polymorphisms induce apoptosis in non-mammals?
dc.typeArticle

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