Immobilization of catalase on chitosan and amino acid-modified chitosan beads
| dc.contributor.author | Basak, E | |
| dc.contributor.author | Aydemir, T | |
| dc.date.accessioned | 2025-04-10T10:26:40Z | |
| dc.date.available | 2025-04-10T10:26:40Z | |
| dc.description.abstract | Bovine liver catalase was covalently immobilized onto amino acid-modified chitosan beads. The beads were characterized with SEM, FTIR, TGA and the effects of immobilization on optimum pH and temperature, thermostability, reusability were evaluated. Immobilized catalase showed the maximal enzyme activity at pH 7.0 at 30 degrees C. The kinetic parameters, K-m and V-max, for immobilized catalase on alanine-chitosan beads and lysine-chitosan beads were estimated to be 25.67 mM, 27 mM and 201.39 mu mol H2O2/min, 197.50 mu mol H2O2/min, respectively. The activity of the immobilized catalase on Ala-CB and Lys-CB retained 40% of its high initial activity after 100 times of reuse. | |
| dc.identifier.issn | 2169-1401 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14701/34306 | |
| dc.language.iso | English | |
| dc.title | Immobilization of catalase on chitosan and amino acid-modified chitosan beads | |
| dc.type | Article |