Investigation of Stem Cell Applications on In Vitro Fertilization in Rats

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dc.contributor.authorGumurdu A.
dc.contributor.authorOzturk S.
dc.contributor.authorAydemir I.
dc.contributor.authorTuglu M.I.
dc.date.accessioned2024-07-22T08:06:23Z
dc.date.available2024-07-22T08:06:23Z
dc.date.issued2021
dc.description.abstractWe aimed to search the effects of bone marrow-derived mesenchymal stem cell-conditioned media on in vitro fertilization by investigation of lifetime of germ cells cleavage, degeneration rates and embryo quality. For this purpose, firstly MSCs were isolated from femurs and tibias of the rat, and cells were cultured until the fourth passage. Sperm and oocytes were collected from male and female rats. Oocytes were added in Human Tubal Fluid Media (HTFM), Single Step Media (SSM), Alpha-MEM Media (AMM) and Bone Marrow-Derived Mesenchymal Stem Cell-Conditioned Media (CM). Thousand sperm were added into the media which including oocytes. Embryos were allowed to produce by IVF. The development of the embryos was followed until the 11th day, and the arrest, degeneration rates and alive embryos were established. The embryos reached 2, 4, 8, 16 cells stages and morula stage in the CM. While AMM had a negative effect on fertilization and embryo development, the most favourable effect was shown to be caused by CM in comparison with the other medias. These results have shown that the beneficial effects of CM in IVF would be a significant increase in the rate of fertility and development of embryos © 2021 A GUMURDU, S OZTURK, I AYDEMIR, MI TUGLU
dc.identifier.DOI-ID10.12681/JHVMS.28489
dc.identifier.issn17922720
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13487
dc.language.isoEnglish
dc.publisherHellenic Veterinary Medical Society
dc.rightsAll Open Access; Hybrid Gold Open Access
dc.subjectamphotericin
dc.subjectcell surface protein
dc.subjectchorionic gonadotropin
dc.subjectendoglin
dc.subjectfollitropin
dc.subjectgentamicin
dc.subjectglutamine
dc.subjectketamine
dc.subjectpenicillin derivative
dc.subjectstreptomycin
dc.subjectstro 1 antigen
dc.subjectunclassified drug
dc.subjectxylazine
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectArticle
dc.subjectbone marrow mesenchymal stem cell
dc.subjectcell culture
dc.subjectcell division
dc.subjectcell isolation
dc.subjectcell structure
dc.subjectcumulus cell
dc.subjectdegeneration
dc.subjectembryo
dc.subjectembryo development
dc.subjectestrus cycle
dc.subjectfemale
dc.subjectfemur
dc.subjectfertility
dc.subjectfetal bovine serum
dc.subjectimmunocytochemistry
dc.subjectimmunoreactivity
dc.subjectin vitro fertilization
dc.subjectmale
dc.subjectmetaphase
dc.subjectmicroscopy
dc.subjectmorula
dc.subjectnonhuman
dc.subjectoocyte
dc.subjectoocyte maturation
dc.subjectovary
dc.subjectovary follicle cell
dc.subjectpregnancy rate
dc.subjectprophase
dc.subjectrat
dc.subjectsperm
dc.subjectspermatocyte
dc.subjectstem cell
dc.subjectstereomicroscopy
dc.subjectsuperovulation
dc.subjecttestis tissue
dc.subjecttibia
dc.subjectvagina smear
dc.titleInvestigation of Stem Cell Applications on In Vitro Fertilization in Rats
dc.typeArticle

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