Apoptosis of colon cancer cells under the effect of geldanamycin derivate

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dc.contributor.authorKosova F.
dc.contributor.authorKasar Z.
dc.contributor.authorTuglu I.
dc.contributor.authorOzdal Kurt F.
dc.contributor.authorGok S.
dc.contributor.authorAri Z.
dc.contributor.authorImren T.
dc.date.accessioned2024-07-22T08:11:03Z
dc.date.available2024-07-22T08:11:03Z
dc.date.issued2017
dc.description.abstractAIM: The apoptotic effect of geldanamycin derivative may be important for the colorectal cancer therapy. The mechanisms of apoptosis require understanding of the behavior of colon cancer cell line Colo-205 which mimics colon adenocarcinoma. Therefore, the effect of IC50 dose of 17-allylamino-17-demethoxygeldanamycin (17- AAG) on the colon cancer cells in vitro was studied for its anti-apoptotic activity. METHOD: Apoptotic ratio of the Colo-205 cells was determined after 17-AAG application with terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining and apoptosis related genes. Apoptosis signal path related key mitochondrial proteins, cytochrome c, bcl-2, caspase 9 and Apaf-1 expression were examined with RT-PCR method. RESULTS: 17-AAG caused induction of cell death. Apoptotic related genes such as cytochrome-c, Apaf-1 and caspase-9 protein expressions were increased signifi cantly (p < 0.05) and anti-apoptotic bcl-2 expression was decreased signifi cantly (p < 0.05). Our results indicated that the application of 17-AAG on Colo-205 cells showed anticancer effect by the apoptosis due to alteration of apoptotic genes. CONCLUSION: The apoptotic effect of 17-AAG as an natural product for alternative medicine would be very important for the success and quality of life during the treatment of colon carcinoma with the combination of anticancer drugs.
dc.identifier.DOI-ID10.4149/BLL_2017_058
dc.identifier.issn00069248
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15473
dc.language.isoEnglish
dc.publisherComenius University
dc.rightsAll Open Access; Bronze Open Access; Green Open Access
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectBenzoquinones
dc.subjectColonic Neoplasms
dc.subjectHumans
dc.subjectLactams, Macrocyclic
dc.subjectTumor Cells, Cultured
dc.subjectantineoplastic agent
dc.subjectbenzoquinone derivative
dc.subjectgeldanamycin
dc.subjectmacrocyclic lactam
dc.subjectapoptosis
dc.subjectColonic Neoplasms
dc.subjectdrug effects
dc.subjecthuman
dc.subjectpathology
dc.subjecttumor cell culture
dc.titleApoptosis of colon cancer cells under the effect of geldanamycin derivate
dc.typeArticle

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