Quantitative approach to lectin-based glycoprofiling of thymic tissues in the control- and the dexamethasone-treated mice

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dc.contributor.authorBalcan E.
dc.date.accessioned2024-07-22T08:11:50Z
dc.date.available2024-07-22T08:11:50Z
dc.date.issued2016
dc.description.abstractDexamethasone (DEX) is the most commonly used synthetic glucocorticoid in treatment of various inflammatory conditions. Here we focused on evaluating the effect of DEX on apoptosis and glycan profile in the mouse thymic tissues. Histological examinations revealed that the DEX treatment cause severe alterations in thymus, such as disruption of thymic capsule, impaired epithelial cell-thymocyte contacts, cellular loss and increased apoptosis. The identification of thymic glycans in the control- and the DEX-treated mice was carried out by using a panel of five plant lectins, Maackia amurensis agglutinin (MAA), peanut agglutinin (PNA), Sambucus nigra agglutinin (SNA), Concanavalin A (ConA) and wheat germ agglutinin (WGA). Lectin histochemistry results showed that glycosylation pattern of thymus changes upon DEX treatment. For further detailed quantitative analyses of the binding intensities for each lectin, histochemical data were scored as high positive (HP), mild positive (MP) and low positive (LP) and differences among signaling densities were investigated. The staining patterns of thymic regions observed with lectin histochemistry suggest that DEX can affect the thymic glycan profile as well as thymocyte apoptosis. These results are consistent with the opinion that not only sialic acid, but also other sugar motifs may be responsible for thymocyte development. © 2016 Elsevier Ltd.
dc.identifier.DOI-ID10.1016/j.tice.2016.03.010
dc.identifier.issn00408166
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/15783
dc.language.isoEnglish
dc.publisherChurchill Livingstone
dc.subjectAnimals
dc.subjectApoptosis
dc.subjectConcanavalin A
dc.subjectDexamethasone
dc.subjectGlycosylation
dc.subjectMice
dc.subjectPeanut Agglutinin
dc.subjectPhytohemagglutinins
dc.subjectPlant Lectins
dc.subjectPolysaccharides
dc.subjectRibosome Inactivating Proteins
dc.subjectThymocytes
dc.subjectThymus Gland
dc.subjectWheat Germ Agglutinins
dc.subjectdexamethasone
dc.subjectglycan
dc.subjectconcanavalin A
dc.subjectdexamethasone
dc.subjectleukoagglutinins, plants
dc.subjectpeanut agglutinin
dc.subjectphytohemagglutinin
dc.subjectplant lectin
dc.subjectpolysaccharide
dc.subjectribosome inactivating protein
dc.subjectSambucus nigra lectins
dc.subjectwheat germ agglutinin
dc.subjectanimal experiment
dc.subjectanimal tissue
dc.subjectapoptosis
dc.subjectArticle
dc.subjectbinding affinity
dc.subjectcarbohydrate analysis
dc.subjectcell loss
dc.subjectcontrolled study
dc.subjectglycoprofiling
dc.subjectglycosylation
dc.subjecthistochemistry
dc.subjecthistology
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectquantitative analysis
dc.subjectthymus
dc.subjectanimal
dc.subjectchemistry
dc.subjectdrug effects
dc.subjectgrowth, development and aging
dc.subjectmetabolism
dc.subjectthymocyte
dc.titleQuantitative approach to lectin-based glycoprofiling of thymic tissues in the control- and the dexamethasone-treated mice
dc.typeArticle

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