Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus

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dc.contributor.authorPala H.G.
dc.contributor.authorPala E.E.
dc.contributor.authorArtunc Ulkumen B.
dc.contributor.authorErbas O.
dc.date.accessioned2024-07-22T08:05:22Z
dc.date.available2024-07-22T08:05:22Z
dc.date.issued2021
dc.description.abstractAim: To study (1) ovarian and endometrial damage caused by the hyperglycemia and (2) the effects of dichloroacetic acid (DCA) on follicular reserve and endometrial damage in streptozocin induced diabetic rats. Methods: This study consisted 24 rats randomly separated into three groups. A diabetes model was achieved in 16 rats experimentally, and normoglycemic eight rats were assigned as control group (Group 1). The rats with diabetes were randomly separated to two groups: 1 mL/kg/day intraperitoneal 0.9% NaCl was given to eight rats as diabetic vehicle (Group 2) and 10 mg/kg/day DCA was given to other eight rats as DCA treated group (Group 3). Hysterectomy with bilateral oophorectomy was performed for histopathological evaluation and blood samples were collected after 4 weeks. Results: Diabetes caused ovarian and endometrial damage (p < 0.0001). Pentraxin-3 (PTX-3), lactic acid, and transforming growth factor-beta (TGF-β) were higher (p < 0.05, p < 0.05, and p < 0.0001, respectively), whereas anti-Mullerian hormone (AMH) was lower in diabetic rats (p < 0.05). These findings reflected the diabetic damage in the genital tract and diminished ovarian reserve occurred via fibrosis, severe inflammation, and oxidative stress. DCA improved the histopathological fibrosis and degeneration in the ovaries and endometrium (p < 0.05). There was a concominant decrease of TGF-β and lactic acid levels with DCA treatment (p < 0.05). DCA also improved ovarian reserve with higher AMH levels (p < 0.05). Conclusions: The several unfavored changes in the endometrium and ovaries due to diabetes have been determined in this present study. DCA might provide the continuity of the endometrial cycle, physiological endometrial structure, ovarian follicular growth, oocyte maturation, and physiological ovarian function by decreasing the lactate levels via inhibiting pyruvate dehydrogenase kinase enzyme. © 2021 Japan Society of Obstetrics and Gynecology.
dc.identifier.DOI-ID10.1111/jog.15045
dc.identifier.issn13418076
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/13109
dc.language.isoEnglish
dc.publisherJohn Wiley and Sons Inc
dc.subjectAnimals
dc.subjectAnti-Mullerian Hormone
dc.subjectDiabetes Mellitus, Experimental
dc.subjectDichloroacetic Acid
dc.subjectEndometrium
dc.subjectFemale
dc.subjectOvarian Reserve
dc.subjectRats
dc.subjectdichloroacetic acid
dc.subjectglucose
dc.subjecthemoglobin A1c
dc.subjectlactic acid
dc.subjectMuellerian inhibiting factor
dc.subjectpentraxin 3
dc.subjectpyruvate dehydrogenase kinase
dc.subjecttransforming growth factor beta
dc.subjectdichloroacetic acid
dc.subjectMuellerian inhibiting factor
dc.subjectArticle
dc.subjectblood sampling
dc.subjectcontrolled study
dc.subjectdrug effect
dc.subjectendometrial disease
dc.subjectendometrium
dc.subjectexperimental diabetes mellitus
dc.subjectfemale
dc.subjectfibrosis
dc.subjectgenital system
dc.subjectglucose blood level
dc.subjecthistopathology
dc.subjecthyperglycemia
dc.subjecthysterectomy
dc.subjectinflammation
dc.subjectmenstrual cycle
dc.subjectnonhuman
dc.subjectoocyte maturation
dc.subjectovarian reserve
dc.subjectovariectomy
dc.subjectoxidative stress
dc.subjectpractice guideline
dc.subjectprotein blood level
dc.subjectprotein expression
dc.subjectrat
dc.subjectanimal
dc.subjectexperimental diabetes mellitus
dc.titleProtective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus
dc.typeArticle

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