Molecular prevalence and genotypes of Enterocytozoon bieneusi in cancer patients under chemotherapy in Aegean region of Türkiye

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dc.contributor.authorAksoy Gökmen A.
dc.contributor.authorÖncü Öner T.
dc.contributor.authorErkunt Alak S.
dc.contributor.authorKoçkaya E.S.
dc.contributor.authorGüvendi M.
dc.contributor.authorKarabey M.
dc.contributor.authorAlacacıoğlu A.
dc.contributor.authorPektaş B.
dc.contributor.authorDeğirmenci Döşkaya A.
dc.contributor.authorKarakavuk M.
dc.contributor.authorDöşkaya M.
dc.contributor.authorÜn C.
dc.contributor.authorGürüz A.Y.
dc.contributor.authorKaya S.
dc.contributor.authorCan H.
dc.date.accessioned2024-07-22T08:01:02Z
dc.date.available2024-07-22T08:01:02Z
dc.date.issued2024
dc.description.abstractBackground: Enterocytozoon bieneusi is the most common species found in humans. Although E. bieneusi has been investigated in humans, genotype profile of E. bieneusi is not known in Türkiye. Methods: In this study, we screened E. bieneusi in patients (n = 94) with different types of malignant solid tumors by Real Time PCR and then sequenced E. bieneusi positive samples. All cancer patients were undergoing chemotherapy and had diarrhea. Moreover, as control groups, we also screened E. bieneusi in patients with diarrhea (n = 50) and without diarrhea (n = 50). Results: Among all patients analyzed, 33 (17%) were found to be E. bieneusi-positive. As the patients were categorized, the molecular prevalence of E. bieneusi increased to 25.5% among cancer patients with diarrhea. However, the molecular prevalence of E. bieneusi was found to be lower in patients with presenting only diarrhea (8%) and patients without diarrhea (10%). The high molecular prevalence value detected among cancer patients with diarrhea was also statistically significant compared to other patient groups (P = 0.00112 and P = 0.0269). Among the 33 Real Time PCR positive samples, 10 of them were amplified by nested PCR and among these 10 samples, 6 of them were successfully genotyped. The phylogenetic tree showed the presence of D and Type IV which were also identified in stray cats living in İzmir in our previous study. Conclusions: High molecular prevalence value indicates the importance of screening stool samples of cancer patients with diarrhea for E. bieneusi and genotyping results indicate that D and Type IV are circulating between humans and cats. © The Author(s) 2024.
dc.identifier.DOI-ID10.1186/s12866-024-03369-3
dc.identifier.issn14712180
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/11292
dc.language.isoEnglish
dc.publisherBioMed Central Ltd
dc.rightsAll Open Access; Gold Open Access
dc.subjectAdult
dc.subjectAged
dc.subjectAged, 80 and over
dc.subjectAntineoplastic Agents
dc.subjectDiarrhea
dc.subjectDNA, Fungal
dc.subjectEnterocytozoon
dc.subjectFeces
dc.subjectFemale
dc.subjectGenotype
dc.subjectHumans
dc.subjectMale
dc.subjectMicrosporidiosis
dc.subjectMiddle Aged
dc.subjectNeoplasms
dc.subjectPhylogeny
dc.subjectPrevalence
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectSequence Analysis, DNA
dc.subjectYoung Adult
dc.subjectDNA polymerase
dc.subjectantineoplastic agent
dc.subjectfungal DNA
dc.subjectadult
dc.subjectArticle
dc.subjectcancer chemotherapy
dc.subjectcancer patient
dc.subjectcat
dc.subjectCD4+ T lymphocyte
dc.subjectcontrolled study
dc.subjectdiarrhea
dc.subjectEnterocytozoon bieneusi
dc.subjectfemale
dc.subjectgenotype
dc.subjectgenotyping
dc.subjecthuman
dc.subjecthydrolysis
dc.subjectimmune system
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmiddle aged
dc.subjectnonhuman
dc.subjectphylogenetic tree
dc.subjectphylogeny
dc.subjectprevalence
dc.subjectreal time polymerase chain reaction
dc.subjectsolid malignant neoplasm
dc.subjectaged
dc.subjectcomplication
dc.subjectdiarrhea
dc.subjectDNA sequencing
dc.subjectdrug therapy
dc.subjectEnterocytozoon
dc.subjectepidemiology
dc.subjectfeces
dc.subjectgenetics
dc.subjectisolation and purification
dc.subjectmicrobiology
dc.subjectmicrosporidiosis
dc.subjectneoplasm
dc.subjectprevalence
dc.subjectvery elderly
dc.subjectveterinary medicine
dc.subjectyoung adult
dc.titleMolecular prevalence and genotypes of Enterocytozoon bieneusi in cancer patients under chemotherapy in Aegean region of Türkiye
dc.typeArticle

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