Analysis of transferred keratinocyte-like cells derived from mouse embryonic stem cells on experimental surgical skin wounds of mouse

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dc.contributor.authorVatansever H.S.
dc.contributor.authorUluer E.T.
dc.contributor.authorAydede H.
dc.contributor.authorOzbilgin M.K.
dc.date.accessioned2024-07-22T08:19:07Z
dc.date.available2024-07-22T08:19:07Z
dc.date.issued2013
dc.description.abstractAutologous/allogenic skin grafts constituted from differentiated adult or embryonic stem cells can be used in treatment of skin disorders. In our study we aimed to differentiate keratinocytes from mouse embryonic stem cells and the transfer of viable keratinocyte-like cells to a model of surgical skin wound of mouse. Embryoid bodies, derived from mouse embryonic stem cells, were cultured on basement membrane matrix with added BMP-4 for 10 days. The identification of differentiated keratinocyte-like cells was done by detection of cytokeratin-8 and cytokeratin-14 localization using an indirect immunoperoxidase technique and transmission electron microscopy evaluation. Distribution of BrdU, cytokeratin-8 and cytokeratin-14 were evaluated using an indirect immunoperoxidase technique from the experimental (dressing including BrdU labelled cells applied after the surgical wound was created on mouse), control (only the surgical wound was created on mouse) and sham (only the dressing applied after the surgical wound was created on mouse) in groups after 3, 5 and 7 days. Immunohistochemically and ultrastructurally, cells derived from mouse embryonic stem cells were similar to differentiated keratinocyte-like cells. Differentiated keratinocyte-like cells were demonstrated by positive BrdU, cytokeratin-8 and cytokeratin-14 staining after transfer to the wound area. In the experimental group wound healing was better after transferring differentiated keratinocytes when compared to the sham and control groups. In vivo continuity and usability of derived cells are very important issues. In wound repair mechanisms, keratinocyte-like cells could provide positive effects during the wound healing and could be used in clinical treatments of wound repair process. © 2012 Elsevier GmbH.
dc.identifier.DOI-ID10.1016/j.acthis.2012.03.004
dc.identifier.issn16180372
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17540
dc.language.isoEnglish
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectDermatologic Surgical Procedures
dc.subjectEmbryonic Stem Cells
dc.subjectKeratinocytes
dc.subjectMale
dc.subjectMice
dc.subjectMice, Inbred BALB C
dc.subjectWound Healing
dc.subjectbone morphogenetic protein 4
dc.subjectcytokeratin 14
dc.subjectcytokeratin 8
dc.subjectperoxidase
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectarticle
dc.subjectbasement membrane
dc.subjectbiotinylation
dc.subjectcell differentiation
dc.subjectcell function
dc.subjectcell proliferation
dc.subjectcell transfer
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectembryo
dc.subjectembryoid body
dc.subjectembryonic stem cell
dc.subjectimmunohistochemistry
dc.subjectimmunolocalization
dc.subjectimmunoreactivity
dc.subjectkeratinocyte
dc.subjectmale
dc.subjectmicroscopy
dc.subjectmouse
dc.subjectneutrophil
dc.subjectnonhuman
dc.subjectskin injury
dc.subjecttransmission electron microscopy
dc.subjectwound healing
dc.titleAnalysis of transferred keratinocyte-like cells derived from mouse embryonic stem cells on experimental surgical skin wounds of mouse
dc.typeArticle

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