Analysis of the effects of inhibitor and activator systems (smad's proteins) of TGF-βs on chick neural tube closure; [Tavuk Nöral Tüp Kapanmasinda TGF-βs Inhibitör ve Aktivatör Sisteminin (Smad's Proteinleri) Etkilerinin Araştirilmasi]
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Date
2010
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Abstract
The families of TGF-βs and Smads proteins that controls its intracellular signaling pathways are known to play a role in early neurulation. The aim of this study is to demonstrate distribution of TGF-βs (1, 2, 3) and Smads (1/2/3, 6, 7) proteins as a system in different hours of neural tube development of chick embryos. The SPF eggs were incubated at 37.8±2°C for 24th, 30th, 48th, 72nd h. After that, embryos were examined using immunohistochemistry and western blotting techniques. To the results, TGF-βs immunoreactivities (particularly TGF-β3) at the 24th, 30th and 48th h of chick development (during neural tube closure) were determined and decreased at the 72nd h (after neural tube closure), but expressions of TGF-βs were detected in all stage of embryos in western blotting. While Smad 1/2/3 immunoreactivitiy and expression was less than that of the Smad 6 and 7 at the 24th, it was increased at the 30th h. Smads proteins immunoreactivities were decresead at the 72nd h. In conclusion, the members of TGF-βs are play a role in chick neural tube closure, the secretions of TGF-βs are controlled different Smad proteins. In addition, immunoblotting results showed that TGF-βs and Smads proteins were effective in the development of all tissues and organs of the embryos.