The use of CRISPR-Cas-based systems in bacterial cell factories

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dc.contributor.authorKolasinliler G.
dc.contributor.authorAagre M.M.
dc.contributor.authorAkkale C.
dc.contributor.authorKaya H.B.
dc.date.accessioned2024-07-22T08:02:53Z
dc.date.available2024-07-22T08:02:53Z
dc.date.issued2023
dc.description.abstractClustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas system has been the focus since its first introduction to genome editing research. It acts as an adaptive immune system in many prokaryotes by detecting and degrading viral/invasive DNA/RNA repurposed for targeted genome editing in various organisms, including bacterial species relevant to industrial biotechnology. Targeted genome editing in bacteria has been implemented for multiple purposes, such as method development, understanding knowledge of metabolic processes and improving the productivity of industrial products. With the increasing share of biotechnological products, bacteria have been the subject in the field of genome editing. This review summarizes the immunological mechanisms of CRISPR-Cas systems, the variation in classification of CRISPR-Cas systems by highlighting their features and the current applications of CRISPR-based genome editing in industrially relevant bacteria. In addition, an extensive literature review focused on recent advances has been presented in bacterial species according to industrial significance. © 2023 Elsevier B.V.
dc.identifier.DOI-ID10.1016/j.bej.2023.108880
dc.identifier.issn1369703X
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/12042
dc.language.isoEnglish
dc.publisherElsevier B.V.
dc.subjectBiotechnology
dc.subjectGenes
dc.subjectAdaptive immune systems
dc.subjectBacterial cells
dc.subjectBacterial species
dc.subjectCas protein
dc.subjectClustered regularly interspaced short palindromic repeat
dc.subjectGenome editing
dc.subjectIndustrial biotechnology
dc.subjectIndustrial production
dc.subjectMethod development
dc.subjectPalindromic
dc.subjectadaptive immunity
dc.subjectbacterial cell
dc.subjectbiotechnology
dc.subjectclustered regularly interspaced short palindromic repeat
dc.subjectCRISPR Cas system
dc.subjectDNA RNA hybridization
dc.subjectfactory
dc.subjectgene editing
dc.subjectindustrial production
dc.subjectmetabolism
dc.subjectnonhuman
dc.subjectproductivity
dc.subjectprokaryote
dc.subjectreview
dc.subjectBacteria
dc.titleThe use of CRISPR-Cas-based systems in bacterial cell factories
dc.typeReview

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