Inhibitory effect of N-acetyl cysteine and ascorbic acid on the development of myringosclerosis: AN experimental study

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dc.contributor.authorDündar R.
dc.contributor.authorInan S.
dc.contributor.authorMuluk N.B.
dc.contributor.authorCingi C.
dc.contributor.authorIlknur A.E.
dc.contributor.authorKatilmiş H.
dc.date.accessioned2024-07-22T08:17:26Z
dc.date.available2024-07-22T08:17:26Z
dc.date.issued2014
dc.description.abstractObjectives: This study investigated the effects of ascorbic acid and N-acetyl cysteine (NAC) antioxidants on the development of myringosclerosis (MS) in an experimental model. Methods: Myringotomies were performed in the ears of 15 guinea pigs, and Spongostan® pieces were placed on the perforated regions of the tympanic membrane. The subjects were divided randomly into three groups and treated with three different solutions on the Spongostan-group 1: (control, 0.9% saline), group 2 (ascorbic acid), and group 3 (NAC). On day 15 after treatment, specimens from the tympanic membranes were obtained and examined via light microscopy. Sclerosis and inflammation scores and the tympanic membrane thicknesses were evaluated. Immunohistochemical methods were used to evaluate the expression of VEGF, TGF-β, iNOS, and IL1-β in all groups. Results: Lower sclerosis and inflammation scores and reduced tympanic membrane thicknesses were observed in groups treated with NAC or ascorbic acid compared with the control group. Immunohistochemical studies revealed significantly less expression of VEGF, TGF-β, and iNOS in groups 2 and 3 compared with group 1. Additionally, IL1-β expression was significantly less in group 3 than in group 1. Compared with group 1, group 2 animals exhibited reduced inflammation in the lamina propria, fewer active fibroblasts, less leukocyte infiltration, and decreased thickness of the vessels; group 3 animals exhibited decreased numbers of active fibroblasts and collagen fibers in the lamina propria. Conclusions: Inflammation scores, cellular infiltration, and expression of VEGF, TGF-β, and iNOS were reduced by ascorbic acid and/or NAC treatments, thereby decreasing MS development. Decreased expression of IL1-β was observed only in animals treated with NAC. © 2014 Elsevier Ireland Ltd.
dc.identifier.DOI-ID10.1016/j.ijporl.2014.03.029
dc.identifier.issn01655876
dc.identifier.urihttp://akademikarsiv.cbu.edu.tr:4000/handle/123456789/17059
dc.language.isoEnglish
dc.publisherElsevier Ireland Ltd
dc.subjectAcetylcysteine
dc.subjectAnimals
dc.subjectAntioxidants
dc.subjectAscorbic Acid
dc.subjectDisease Models, Animal
dc.subjectFemale
dc.subjectFibrin Foam
dc.subjectFibroblasts
dc.subjectFree Radical Scavengers
dc.subjectGuinea Pigs
dc.subjectHemostatics
dc.subjectImmunohistochemistry
dc.subjectInflammation
dc.subjectLeukocytes
dc.subjectMicroscopy
dc.subjectMucous Membrane
dc.subjectMyringosclerosis
dc.subjectNitric Oxide Synthase
dc.subjectRandom Allocation
dc.subjectTransforming Growth Factor beta
dc.subjectTympanic Membrane
dc.subjectVascular Endothelial Growth Factor A
dc.subjectacetylcysteine
dc.subjectascorbic acid
dc.subjectinducible nitric oxide synthase
dc.subjectinterleukin 1beta
dc.subjectsodium chloride
dc.subjectspongostan
dc.subjecttransforming growth factor beta
dc.subjectvasculotropin
dc.subjectacetylcysteine
dc.subjectantioxidant
dc.subjectascorbic acid
dc.subjectfibrin
dc.subjecthemostatic agent
dc.subjectnitric oxide synthase
dc.subjectscavenger
dc.subjecttransforming growth factor beta
dc.subjectvasculotropin A
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectantioxidant activity
dc.subjectarticle
dc.subjectcell infiltration
dc.subjectcontrolled study
dc.subjectdisease course
dc.subjectfemale
dc.subjectfibroblast
dc.subjectimmunohistochemistry
dc.subjectinflammation
dc.subjectlamina propria
dc.subjectmicroscopy
dc.subjectmyringosclerosis
dc.subjectmyringotomy
dc.subjectnonhuman
dc.subjectpathogenesis
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectrat
dc.subjectsclerosis
dc.subjectscoring system
dc.subjectthickness
dc.subjecttympanic membrane thickness
dc.subjectanimal
dc.subjectdisease model
dc.subjecteardrum
dc.subjectguinea pig
dc.subjectleukocyte
dc.subjectmetabolism
dc.subjectmucosa
dc.subjectmyringosclerosis
dc.subjectpathology
dc.subjectrandomization
dc.titleInhibitory effect of N-acetyl cysteine and ascorbic acid on the development of myringosclerosis: AN experimental study
dc.typeArticle

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